Acquisition, Processing and Analysis of Electrocardiogram in Awake Zebrafish

Tai Le, Michael Lenning, Isaac Clark, Ishaan Bhimani, Joseph Fortunato, Paul Marsh, Xiaolei Xu, Hung Cao

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Long-term monitoring of intrinsic electrocardiogram (ECG) in zebrafish plays a crucial role in heart disease studies and drug screening. In this paper, we developed a polymer-based apparatus with embedded flexible thin-film electrodes to acquire ECG signals of awake zebrafish. The apparatus was made of polydimethylsiloxane using the molding technique with molds formed by 3D printing. A graphical user interface was built in National Instruments LabView platform for real-time recording, processing, and analysis. The program provided important features, such as signal de-noising, characteristic wave detection, and anomaly detection. Further, it could operate on both real-time coming signals and previously-saved data, facilitating analysis and interpretation. We demonstrated the use of our system to investigate the effects of the anesthetic drug, namely Tricaine (MS-222), on cardiac electrophysiology of zebrafish, revealing promising findings. We speculate that our novel system may contribute to a host of studies in various disciplines using the zebrafish model.

Original languageEnglish (US)
Article number8636511
Pages (from-to)4283-4289
Number of pages7
JournalIEEE Sensors Journal
Volume19
Issue number11
DOIs
StatePublished - Jun 1 2019

Keywords

  • Zebrafish
  • automated processing
  • electrocardiogram (ECG)
  • heart disease
  • labview

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

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    Le, T., Lenning, M., Clark, I., Bhimani, I., Fortunato, J., Marsh, P., Xu, X., & Cao, H. (2019). Acquisition, Processing and Analysis of Electrocardiogram in Awake Zebrafish. IEEE Sensors Journal, 19(11), 4283-4289. [8636511]. https://doi.org/10.1109/JSEN.2019.2897789