Acquisition, Processing and Analysis of Electrocardiogram in Awake Zebrafish

Tai Le, Michael Lenning, Isaac Clark, Ishaan Bhimani, Joseph Fortunato, Paul Marsh, Xiaolei H Xu, Hung Cao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Long-term monitoring of intrinsic electrocardiogram (ECG) in zebrafish plays a crucial role in heart disease studies and drug screening. In this paper, we developed a polymer-based apparatus with embedded flexible thin-film electrodes to acquire ECG signals of awake zebrafish. The apparatus was made of polydimethylsiloxane using the molding technique with molds formed by 3D printing. A graphical user interface was built in National Instruments LabView platform for real-time recording, processing, and analysis. The program provided important features, such as signal de-noising, characteristic wave detection, and anomaly detection. Further, it could operate on both real-time coming signals and previously-saved data, facilitating analysis and interpretation. We demonstrated the use of our system to investigate the effects of the anesthetic drug, namely Tricaine (MS-222), on cardiac electrophysiology of zebrafish, revealing promising findings. We speculate that our novel system may contribute to a host of studies in various disciplines using the zebrafish model.

Original languageEnglish (US)
Article number8636511
Pages (from-to)4283-4289
Number of pages7
JournalIEEE Sensors Journal
Volume19
Issue number11
DOIs
StatePublished - Jun 1 2019

Fingerprint

electrocardiography
Electrocardiography
acquisition
drugs
electrophysiology
Signal denoising
heart diseases
anesthetics
Anesthetics
graphical user interface
time signals
Molds
Polydimethylsiloxane
Graphical user interfaces
Processing
Molding
printing
Printing
Screening
screening

Keywords

  • automated processing
  • electrocardiogram (ECG)
  • heart disease
  • labview
  • Zebrafish

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Le, T., Lenning, M., Clark, I., Bhimani, I., Fortunato, J., Marsh, P., ... Cao, H. (2019). Acquisition, Processing and Analysis of Electrocardiogram in Awake Zebrafish. IEEE Sensors Journal, 19(11), 4283-4289. [8636511]. https://doi.org/10.1109/JSEN.2019.2897789

Acquisition, Processing and Analysis of Electrocardiogram in Awake Zebrafish. / Le, Tai; Lenning, Michael; Clark, Isaac; Bhimani, Ishaan; Fortunato, Joseph; Marsh, Paul; Xu, Xiaolei H; Cao, Hung.

In: IEEE Sensors Journal, Vol. 19, No. 11, 8636511, 01.06.2019, p. 4283-4289.

Research output: Contribution to journalArticle

Le, T, Lenning, M, Clark, I, Bhimani, I, Fortunato, J, Marsh, P, Xu, XH & Cao, H 2019, 'Acquisition, Processing and Analysis of Electrocardiogram in Awake Zebrafish', IEEE Sensors Journal, vol. 19, no. 11, 8636511, pp. 4283-4289. https://doi.org/10.1109/JSEN.2019.2897789
Le T, Lenning M, Clark I, Bhimani I, Fortunato J, Marsh P et al. Acquisition, Processing and Analysis of Electrocardiogram in Awake Zebrafish. IEEE Sensors Journal. 2019 Jun 1;19(11):4283-4289. 8636511. https://doi.org/10.1109/JSEN.2019.2897789
Le, Tai ; Lenning, Michael ; Clark, Isaac ; Bhimani, Ishaan ; Fortunato, Joseph ; Marsh, Paul ; Xu, Xiaolei H ; Cao, Hung. / Acquisition, Processing and Analysis of Electrocardiogram in Awake Zebrafish. In: IEEE Sensors Journal. 2019 ; Vol. 19, No. 11. pp. 4283-4289.
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