TY - JOUR
T1 - Acidity is involved in the development of neuropathy caused by oxidized cellulose
AU - Nagamatsu, Masaaki
AU - Podratz, Jewel
AU - Windebank, Anthony J.
AU - Low, Phillip A.
N1 - Funding Information:
Supported in part by grants from NINDS (PO1 NS32352 (PAL), NS22352 (PAL), NS30534 (PAL), NS14304 (AJW)), NASA (PAL), and Mayo Funds (PAL). Appreciation is expressed to James D. Schmelzer for technical support and Anita Zeller for secretarial assistance.
PY - 1997/3/10
Y1 - 1997/3/10
N2 - Recently we demonstrated that oxidized cellulose (OC), a surgical topical hemostatic agent, induces subjacent nerve fiber degeneration by a diffusible chemical mechanism. Since OC is highly acidic, we examined the role of acidity in the development of neuropathy by OC in this study. Fifteen minutes' exposure to culture media containing OC (2 mg/ml, pH 3.47 or 10 mg/ml, pH 2.57) suppressed the subsequent neurite outgrowth of precultured rat DRG neurons in vitro. However, the neurotoxicity of OC disappeared when the pH of the media was restored to 7.42. Topical application of 20 mg OC lowered the pH in the subperineurium of the adjacent rat sciatic nerve to around 3, and kept it below 4 for 2 h in vivo. Application of 0.1 ml neutralized physiological saline containing 40 mg OC did not produce pathological changes in the adjacent rat sciatic nerve in vivo, in contrast to the marked subperineurial nerve damage by direct application of 20 or 40 mg OC observed in our previous study. These results strongly indicate that local neurotoxicity of OC is due to its high acidity. Further care is needed to avoid direct application of large amounts of OC to peripheral nerve.
AB - Recently we demonstrated that oxidized cellulose (OC), a surgical topical hemostatic agent, induces subjacent nerve fiber degeneration by a diffusible chemical mechanism. Since OC is highly acidic, we examined the role of acidity in the development of neuropathy by OC in this study. Fifteen minutes' exposure to culture media containing OC (2 mg/ml, pH 3.47 or 10 mg/ml, pH 2.57) suppressed the subsequent neurite outgrowth of precultured rat DRG neurons in vitro. However, the neurotoxicity of OC disappeared when the pH of the media was restored to 7.42. Topical application of 20 mg OC lowered the pH in the subperineurium of the adjacent rat sciatic nerve to around 3, and kept it below 4 for 2 h in vivo. Application of 0.1 ml neutralized physiological saline containing 40 mg OC did not produce pathological changes in the adjacent rat sciatic nerve in vivo, in contrast to the marked subperineurial nerve damage by direct application of 20 or 40 mg OC observed in our previous study. These results strongly indicate that local neurotoxicity of OC is due to its high acidity. Further care is needed to avoid direct application of large amounts of OC to peripheral nerve.
KW - dorsal root ganglia
KW - hydrogen-ion concentration
KW - hydrogen-ion microelectrode
KW - neuropathy
KW - oxidized cellulose
KW - rat
KW - sciatic nerve
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U2 - 10.1016/S0022-510X(96)00295-X
DO - 10.1016/S0022-510X(96)00295-X
M3 - Article
C2 - 9077504
AN - SCOPUS:0031058313
SN - 0022-510X
VL - 146
SP - 97
EP - 102
JO - Journal of the neurological sciences
JF - Journal of the neurological sciences
IS - 2
ER -