TY - JOUR
T1 - Acetylcholinesterase in Huntington's and Alzheimer's Diseases
T2 - Simultaneous Enzyme Assay and Immunoassay of Multiple Brain Regions
AU - Hammond, Pamela
AU - Brimijoin, Stephen
PY - 1988/4
Y1 - 1988/4
N2 - Abstract: A newJy developed enzyme‐linked immunosorbent assay for acetylcholinesterase (AChE) protein was combined with conventional measures of enzyme activity in a study of 15 brain regions from six control cases (non‐neurological deaths), six cases of Alzheimer's disease, and six cases of Huntington's disease. In the control brains, the mean AChE activity varied 100‐fold from region to region (cortex lowest, striatum highest). The variation in enzyme activity was exactly paralleled by a variation in protein immunoreactivity. Overall, the homospecific activity of AChE averaged 0.26 ± 0.007 mU/pg, close to the value for electrophoretically homogeneous enzyme isolated from red blood cells. Similar homospecific activities were observed in samples from Huntington's and Alzheimer's brains. Evidently, AChE that is immunoreactive but enzymatically inactive does not accumulate in any of the three conditions examined. Huntington's brain samples showed normal total contents of AChE, but Alzheimer's brains showed significant decreases of both enzyme activity and immunore‐activity in all seven cortical regions and in two out of the eight subcortical structures examined, hippocampus and nucleus accumbens.
AB - Abstract: A newJy developed enzyme‐linked immunosorbent assay for acetylcholinesterase (AChE) protein was combined with conventional measures of enzyme activity in a study of 15 brain regions from six control cases (non‐neurological deaths), six cases of Alzheimer's disease, and six cases of Huntington's disease. In the control brains, the mean AChE activity varied 100‐fold from region to region (cortex lowest, striatum highest). The variation in enzyme activity was exactly paralleled by a variation in protein immunoreactivity. Overall, the homospecific activity of AChE averaged 0.26 ± 0.007 mU/pg, close to the value for electrophoretically homogeneous enzyme isolated from red blood cells. Similar homospecific activities were observed in samples from Huntington's and Alzheimer's brains. Evidently, AChE that is immunoreactive but enzymatically inactive does not accumulate in any of the three conditions examined. Huntington's brain samples showed normal total contents of AChE, but Alzheimer's brains showed significant decreases of both enzyme activity and immunore‐activity in all seven cortical regions and in two out of the eight subcortical structures examined, hippocampus and nucleus accumbens.
KW - Acetylcholinesterase
KW - Alzheimer's disease
KW - Enzyme assay
KW - Huntington's disease
KW - Immunoassay
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U2 - 10.1111/j.1471-4159.1988.tb10580.x
DO - 10.1111/j.1471-4159.1988.tb10580.x
M3 - Article
C2 - 2964509
AN - SCOPUS:0023904343
SN - 0022-3042
VL - 50
SP - 1111
EP - 1116
JO - Journal of neurochemistry
JF - Journal of neurochemistry
IS - 4
ER -