Acetylcholine receptor M3 domain: Stereochemical and volume contributions to channel gating

Hai Long Wang, Margherita Milone, Kinji Ohno, Xing Ming Shen, Akira Tsujino, Anna Paola Batocchi, Pietro Tonali, Joan Brengman, Andrew G. Engel, Steven M. Sine

Research output: Contribution to journalArticlepeer-review

102 Scopus citations

Abstract

By defining the functional defect in a congenital myasthenic syndrome (CMS), we show that the third transmembrane domain (M3) of the muscle acetylcholine receptor governs the speed and efficiency of gating of its channel. The clinical phenotype of this CMS results from the mutation V2851 in M3 of the α subunit, which attenuates endplate currents, accelerates their decay and causes abnormally brief acetylcholine-induced single-channel currents. Kinetic analysis of engineered αV2851 receptors demonstrated a predominant effect on channel gating, with abnormally slow opening and rapid closing rates. Analysis of site-directed mutations revealed stereochemical and volume-dependent contributions of αV285 to channel gating. Thus, we demonstrate a functional role for the M3 domain as a key component of the nicotinic acetylcholine receptor channel-gating mechanism.

Original languageEnglish (US)
Pages (from-to)226-233
Number of pages8
JournalNature Neuroscience
Volume2
Issue number3
DOIs
StatePublished - Mar 1999

ASJC Scopus subject areas

  • Neuroscience(all)

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