Acetylation of Histone H3 Lysine 56 Regulates Replication-Coupled Nucleosome Assembly

Qing Li, Hui Zhou, Hugo Wurtele, Brian Davies, Bruce Horazdovsky, Alain Verreault, Zhiguo Zhang

Research output: Contribution to journalArticlepeer-review

298 Scopus citations

Abstract

Chromatin assembly factor 1 (CAF-1) and Rtt106 participate in the deposition of newly synthesized histones onto replicating DNA to form nucleosomes. This process is critical for the maintenance of genome stability and inheritance of functionally specialized chromatin structures in proliferating cells. However, the molecular functions of the acetylation of newly synthesized histones in this DNA replication-coupled nucleosome assembly pathway remain enigmatic. Here we show that histone H3 acetylated at lysine 56 (H3K56Ac) is incorporated onto replicating DNA and, by increasing the binding affinity of CAF-1 and Rtt106 for histone H3, H3K56Ac enhances the ability of these histone chaperones to assemble DNA into nucleosomes. Genetic analysis indicates that H3K56Ac acts in a nonredundant manner with the acetylation of the N-terminal residues of H3 and H4 in nucleosome assembly. These results reveal a mechanism by which H3K56Ac regulates replication-coupled nucleosome assembly mediated by CAF-1 and Rtt106.

Original languageEnglish (US)
Pages (from-to)244-255
Number of pages12
JournalCell
Volume134
Issue number2
DOIs
StatePublished - Jul 25 2008

Keywords

  • DNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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