Accuracy of motor axon regeneration across autograft, single-lumen, and multichannel poly(lactic-co-glycolic acid) nerve tubes

Godard C. De Ruiter, Robert J. Spinner, Martijn J.A. Malessy, Michael J. Moore, Eric J. Sorenson, Bradford L. Currier, Michael J. Yaszemski, Anthony J. Windebank

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

Objective: The accuracy of motor axon regeneration becomes an important issue in the development of a nerve tube for motor nerve repair. Dispersion of regeneration across the nerve tube may lead to misdirection and polyinnervation. In this study, we present a series of methods to investigate the accuracy of regeneration, which we used to compare regeneration across autografts and single-lumen poly(lactic-co-glycolic acid) (PLGA) nerve tubes. We also present the concept of the multichannel nerve tube that may limit dispersion by separately guiding groups of regenerating axons. METHODS: The simultaneous tracing of the tibial and peroneal nerves with fast blue and diamidino yellow was performed 8 weeks after the repair of a 1-cm nerve gap in the rat sciatic nerve to determine the percentage of double-projecting motoneurons. Sequential tracing of the peroneal nerve with diamidino yellow 1 week before repair and fast blue 8 weeks after repair was performed to determine the percentage of correctly directed peroneal motoneurons. RESULTS: In the cases in which there was successful regeneration across single-lumen nerve tubes, more motoneurons had double projections to both the tibial and peroneal nerve branches after single-lumen nerve tube repair (21.4%) than after autograft repair (5.9%). After multichannel nerve tube repair, this percentage was slightly reduced (16.9%), although not significantly. The direction of regeneration was nonspecific after all types of repair. CONCLUSION: Retrograde tracing techniques provide new insights into the process of regeneration across nerve tubes. The methods and data presented in this study can be used as a basis for the development of a nerve tube for motor nerve repair.

Original languageEnglish (US)
Pages (from-to)144-153
Number of pages10
JournalNeurosurgery
Volume63
Issue number1
DOIs
StatePublished - Jul 2008

Keywords

  • Axon targeting
  • Double labeling
  • Misdirection
  • Peripheral nerve regeneration
  • Rat sciatic nerve model
  • Retrograde tracing

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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