Accuracy of cervical specimens obtained for biomarker studies in women with CIN3

Nicolas Wentzensen, Rosemary E. Zuna, Mark E. Sherman, Michael A. Gold, Mark Schiffman, S. Terence Dunn, Jose Jeronimo, Roy Zhang, Joan Walker, Sophia S. Wang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Objective: We developed a protocol to collect representative cervical specimens based on colposcopic evaluation from women treated with loop electrosurgical excision procedure (LEEP). Methods: We analyzed the histology of biopsies targeting the worst and a normal area on the cervical surface in 74 women referred for LEEP because of cervical intraepithelial neoplasia grade 3 (CIN3) detected in a previous biopsy. Lesions and normal tissue were identified in colposcopy, marked, and removed after LEEP. Cervical cytology specimens collected at the same time were analyzed using Pap cytology and human papillomavirus (HPV) genotyping. Results: All but two women had an abnormal colposcopic impression with 59 of 68 (87%) showing an impression of CIN2 or greater. In 19 of 58 (33%) women, the histology result of the frozen specimen targeting the worst lesion was ≤ CIN1. In 18 of 46 (40%) women, the histology of the frozen specimen targeting normal tissue was CIN2+. A concordant histology result in specimens targeting the worst lesion was associated with a greater extension of the CIN3 in the LEEP (p trend = 0.002) and a HSIL cytology result (p trend = 0.02). Conclusion: It is challenging to sample representative cervical tissue. Even in women with confirmed CIN3, colposcopy performance to identify the worst lesion on the cervix was limited. Correctly identified CIN3s were more likely to be larger lesions that may have a higher risk of progression to cancer.

Original languageEnglish (US)
Pages (from-to)493-496
Number of pages4
JournalGynecologic Oncology
Volume115
Issue number3
DOIs
StatePublished - Dec 2009

Fingerprint

Cervical Intraepithelial Neoplasia
Biomarkers
Histology
Cell Biology
Colposcopy
Biopsy
Cervix Uteri
Neoplasms

Keywords

  • Biopsy
  • Cervical cancer
  • Colposcopy
  • Epidemiology
  • HPV
  • Molecular
  • SUCCEED

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Oncology

Cite this

Wentzensen, N., Zuna, R. E., Sherman, M. E., Gold, M. A., Schiffman, M., Dunn, S. T., ... Wang, S. S. (2009). Accuracy of cervical specimens obtained for biomarker studies in women with CIN3. Gynecologic Oncology, 115(3), 493-496. https://doi.org/10.1016/j.ygyno.2009.09.001

Accuracy of cervical specimens obtained for biomarker studies in women with CIN3. / Wentzensen, Nicolas; Zuna, Rosemary E.; Sherman, Mark E.; Gold, Michael A.; Schiffman, Mark; Dunn, S. Terence; Jeronimo, Jose; Zhang, Roy; Walker, Joan; Wang, Sophia S.

In: Gynecologic Oncology, Vol. 115, No. 3, 12.2009, p. 493-496.

Research output: Contribution to journalArticle

Wentzensen, N, Zuna, RE, Sherman, ME, Gold, MA, Schiffman, M, Dunn, ST, Jeronimo, J, Zhang, R, Walker, J & Wang, SS 2009, 'Accuracy of cervical specimens obtained for biomarker studies in women with CIN3', Gynecologic Oncology, vol. 115, no. 3, pp. 493-496. https://doi.org/10.1016/j.ygyno.2009.09.001
Wentzensen, Nicolas ; Zuna, Rosemary E. ; Sherman, Mark E. ; Gold, Michael A. ; Schiffman, Mark ; Dunn, S. Terence ; Jeronimo, Jose ; Zhang, Roy ; Walker, Joan ; Wang, Sophia S. / Accuracy of cervical specimens obtained for biomarker studies in women with CIN3. In: Gynecologic Oncology. 2009 ; Vol. 115, No. 3. pp. 493-496.
@article{03842f030960404e8ab4766c51659555,
title = "Accuracy of cervical specimens obtained for biomarker studies in women with CIN3",
abstract = "Objective: We developed a protocol to collect representative cervical specimens based on colposcopic evaluation from women treated with loop electrosurgical excision procedure (LEEP). Methods: We analyzed the histology of biopsies targeting the worst and a normal area on the cervical surface in 74 women referred for LEEP because of cervical intraepithelial neoplasia grade 3 (CIN3) detected in a previous biopsy. Lesions and normal tissue were identified in colposcopy, marked, and removed after LEEP. Cervical cytology specimens collected at the same time were analyzed using Pap cytology and human papillomavirus (HPV) genotyping. Results: All but two women had an abnormal colposcopic impression with 59 of 68 (87{\%}) showing an impression of CIN2 or greater. In 19 of 58 (33{\%}) women, the histology result of the frozen specimen targeting the worst lesion was ≤ CIN1. In 18 of 46 (40{\%}) women, the histology of the frozen specimen targeting normal tissue was CIN2+. A concordant histology result in specimens targeting the worst lesion was associated with a greater extension of the CIN3 in the LEEP (p trend = 0.002) and a HSIL cytology result (p trend = 0.02). Conclusion: It is challenging to sample representative cervical tissue. Even in women with confirmed CIN3, colposcopy performance to identify the worst lesion on the cervix was limited. Correctly identified CIN3s were more likely to be larger lesions that may have a higher risk of progression to cancer.",
keywords = "Biopsy, Cervical cancer, Colposcopy, Epidemiology, HPV, Molecular, SUCCEED",
author = "Nicolas Wentzensen and Zuna, {Rosemary E.} and Sherman, {Mark E.} and Gold, {Michael A.} and Mark Schiffman and Dunn, {S. Terence} and Jose Jeronimo and Roy Zhang and Joan Walker and Wang, {Sophia S.}",
year = "2009",
month = "12",
doi = "10.1016/j.ygyno.2009.09.001",
language = "English (US)",
volume = "115",
pages = "493--496",
journal = "Gynecologic Oncology",
issn = "0090-8258",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Accuracy of cervical specimens obtained for biomarker studies in women with CIN3

AU - Wentzensen, Nicolas

AU - Zuna, Rosemary E.

AU - Sherman, Mark E.

AU - Gold, Michael A.

AU - Schiffman, Mark

AU - Dunn, S. Terence

AU - Jeronimo, Jose

AU - Zhang, Roy

AU - Walker, Joan

AU - Wang, Sophia S.

PY - 2009/12

Y1 - 2009/12

N2 - Objective: We developed a protocol to collect representative cervical specimens based on colposcopic evaluation from women treated with loop electrosurgical excision procedure (LEEP). Methods: We analyzed the histology of biopsies targeting the worst and a normal area on the cervical surface in 74 women referred for LEEP because of cervical intraepithelial neoplasia grade 3 (CIN3) detected in a previous biopsy. Lesions and normal tissue were identified in colposcopy, marked, and removed after LEEP. Cervical cytology specimens collected at the same time were analyzed using Pap cytology and human papillomavirus (HPV) genotyping. Results: All but two women had an abnormal colposcopic impression with 59 of 68 (87%) showing an impression of CIN2 or greater. In 19 of 58 (33%) women, the histology result of the frozen specimen targeting the worst lesion was ≤ CIN1. In 18 of 46 (40%) women, the histology of the frozen specimen targeting normal tissue was CIN2+. A concordant histology result in specimens targeting the worst lesion was associated with a greater extension of the CIN3 in the LEEP (p trend = 0.002) and a HSIL cytology result (p trend = 0.02). Conclusion: It is challenging to sample representative cervical tissue. Even in women with confirmed CIN3, colposcopy performance to identify the worst lesion on the cervix was limited. Correctly identified CIN3s were more likely to be larger lesions that may have a higher risk of progression to cancer.

AB - Objective: We developed a protocol to collect representative cervical specimens based on colposcopic evaluation from women treated with loop electrosurgical excision procedure (LEEP). Methods: We analyzed the histology of biopsies targeting the worst and a normal area on the cervical surface in 74 women referred for LEEP because of cervical intraepithelial neoplasia grade 3 (CIN3) detected in a previous biopsy. Lesions and normal tissue were identified in colposcopy, marked, and removed after LEEP. Cervical cytology specimens collected at the same time were analyzed using Pap cytology and human papillomavirus (HPV) genotyping. Results: All but two women had an abnormal colposcopic impression with 59 of 68 (87%) showing an impression of CIN2 or greater. In 19 of 58 (33%) women, the histology result of the frozen specimen targeting the worst lesion was ≤ CIN1. In 18 of 46 (40%) women, the histology of the frozen specimen targeting normal tissue was CIN2+. A concordant histology result in specimens targeting the worst lesion was associated with a greater extension of the CIN3 in the LEEP (p trend = 0.002) and a HSIL cytology result (p trend = 0.02). Conclusion: It is challenging to sample representative cervical tissue. Even in women with confirmed CIN3, colposcopy performance to identify the worst lesion on the cervix was limited. Correctly identified CIN3s were more likely to be larger lesions that may have a higher risk of progression to cancer.

KW - Biopsy

KW - Cervical cancer

KW - Colposcopy

KW - Epidemiology

KW - HPV

KW - Molecular

KW - SUCCEED

UR - http://www.scopus.com/inward/record.url?scp=70350575256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350575256&partnerID=8YFLogxK

U2 - 10.1016/j.ygyno.2009.09.001

DO - 10.1016/j.ygyno.2009.09.001

M3 - Article

C2 - 19773045

AN - SCOPUS:70350575256

VL - 115

SP - 493

EP - 496

JO - Gynecologic Oncology

JF - Gynecologic Oncology

SN - 0090-8258

IS - 3

ER -