Absence of strand breaks in deoxyribonucleic acid treated with metronidazole

Nicholas F La Russo, M. Tomasz, D. Kaplan, M. Mueller

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The deoxyribonucleic acid (DNA)-degrading potential of metronidazole was evaluated in vitro by three techniques: determination of melting curve, measurement of viscosity, and centrifugation in neutral or alkaline sucrose gradients. Studies were performed on calf thymus DNA and on 3H-labeled or unlabeled pneumococcal and T7 phage DNA after treatment with metronidazole alone or metronidazole reduced by sodium dithionite in the presence of DNA. This latter process is known to elicit covalent binding of metronidazole to DNA. Reduced or unreduced metronidazole had no effect on the melting properties, viscosity, or sedimentation velocity of the nucleic acids studied. Sodium dithionite alone, however, caused a 25% decrease in the intrinsic viscosity of pneumococcal DNA, and decreased the sedimentation velocity of pneumococcal and T7 phage DNA in both neutral and alkaline sucrose gradients. These data suggest that degradation of DNA is not important in the interaction of metronidazole with nucleic acids, an interaction assumed relevant to the cytotoxic, radiosensitizing, and mutagenic activities of this compound.

Original languageEnglish (US)
Pages (from-to)19-24
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume13
Issue number1
StatePublished - 1978
Externally publishedYes

Fingerprint

Metronidazole
DNA
Viscosity
Dithionite
Bacteriophage T7
Nucleic Acids
Freezing
Sucrose
Centrifugation
Thymus Gland

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Absence of strand breaks in deoxyribonucleic acid treated with metronidazole. / La Russo, Nicholas F; Tomasz, M.; Kaplan, D.; Mueller, M.

In: Antimicrobial Agents and Chemotherapy, Vol. 13, No. 1, 1978, p. 19-24.

Research output: Contribution to journalArticle

@article{84cf77119a454a248c745a234b612f89,
title = "Absence of strand breaks in deoxyribonucleic acid treated with metronidazole",
abstract = "The deoxyribonucleic acid (DNA)-degrading potential of metronidazole was evaluated in vitro by three techniques: determination of melting curve, measurement of viscosity, and centrifugation in neutral or alkaline sucrose gradients. Studies were performed on calf thymus DNA and on 3H-labeled or unlabeled pneumococcal and T7 phage DNA after treatment with metronidazole alone or metronidazole reduced by sodium dithionite in the presence of DNA. This latter process is known to elicit covalent binding of metronidazole to DNA. Reduced or unreduced metronidazole had no effect on the melting properties, viscosity, or sedimentation velocity of the nucleic acids studied. Sodium dithionite alone, however, caused a 25{\%} decrease in the intrinsic viscosity of pneumococcal DNA, and decreased the sedimentation velocity of pneumococcal and T7 phage DNA in both neutral and alkaline sucrose gradients. These data suggest that degradation of DNA is not important in the interaction of metronidazole with nucleic acids, an interaction assumed relevant to the cytotoxic, radiosensitizing, and mutagenic activities of this compound.",
author = "{La Russo}, {Nicholas F} and M. Tomasz and D. Kaplan and M. Mueller",
year = "1978",
language = "English (US)",
volume = "13",
pages = "19--24",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Absence of strand breaks in deoxyribonucleic acid treated with metronidazole

AU - La Russo, Nicholas F

AU - Tomasz, M.

AU - Kaplan, D.

AU - Mueller, M.

PY - 1978

Y1 - 1978

N2 - The deoxyribonucleic acid (DNA)-degrading potential of metronidazole was evaluated in vitro by three techniques: determination of melting curve, measurement of viscosity, and centrifugation in neutral or alkaline sucrose gradients. Studies were performed on calf thymus DNA and on 3H-labeled or unlabeled pneumococcal and T7 phage DNA after treatment with metronidazole alone or metronidazole reduced by sodium dithionite in the presence of DNA. This latter process is known to elicit covalent binding of metronidazole to DNA. Reduced or unreduced metronidazole had no effect on the melting properties, viscosity, or sedimentation velocity of the nucleic acids studied. Sodium dithionite alone, however, caused a 25% decrease in the intrinsic viscosity of pneumococcal DNA, and decreased the sedimentation velocity of pneumococcal and T7 phage DNA in both neutral and alkaline sucrose gradients. These data suggest that degradation of DNA is not important in the interaction of metronidazole with nucleic acids, an interaction assumed relevant to the cytotoxic, radiosensitizing, and mutagenic activities of this compound.

AB - The deoxyribonucleic acid (DNA)-degrading potential of metronidazole was evaluated in vitro by three techniques: determination of melting curve, measurement of viscosity, and centrifugation in neutral or alkaline sucrose gradients. Studies were performed on calf thymus DNA and on 3H-labeled or unlabeled pneumococcal and T7 phage DNA after treatment with metronidazole alone or metronidazole reduced by sodium dithionite in the presence of DNA. This latter process is known to elicit covalent binding of metronidazole to DNA. Reduced or unreduced metronidazole had no effect on the melting properties, viscosity, or sedimentation velocity of the nucleic acids studied. Sodium dithionite alone, however, caused a 25% decrease in the intrinsic viscosity of pneumococcal DNA, and decreased the sedimentation velocity of pneumococcal and T7 phage DNA in both neutral and alkaline sucrose gradients. These data suggest that degradation of DNA is not important in the interaction of metronidazole with nucleic acids, an interaction assumed relevant to the cytotoxic, radiosensitizing, and mutagenic activities of this compound.

UR - http://www.scopus.com/inward/record.url?scp=0017802704&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017802704&partnerID=8YFLogxK

M3 - Article

C2 - 626487

AN - SCOPUS:0017802704

VL - 13

SP - 19

EP - 24

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 1

ER -