Absence of cyclin D1 protein expression in splenic marginal zone lymphoma

Elena Savilo, E. Campo, M. Mollejo, M. Pinyol, M. A. Piris, L. R. Zukerberg, W. I. Yang, D. D. Koelliker, P. L. Nguyen, N. L. Harris

Research output: Contribution to journalArticle

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Abstract

Splenic marginal zone lymphoma (SMZL) is a recently recognized primary splenic lymphoma. SMZL can be associated with peripheral lymphocytosis, in which some cells have a villous morphology (splenic lymphoma with villous lymphocytes [SLVL]). Several recent studies suggested involvement of the bcl- 1 locus and/or the cyclin D1 gene, located on chromosome 11q, in SMZL/SLVL. Translocation t (11;14) is associated with rearrangement of the bcl-1 locus and overexpression of the cyclin D1 gene. Both the translocation and cyclin D1 protein expression are characteristic of mantle cell lymphoma (MCL and are rare in other lymphoid neoplasms; thus, their detection is useful in the diagnosis of MCL. Because the differential diagnosis of low-grade lymphoma in the spleen can be difficult, it is important to assess the frequency of cyclin D1 expression in SMZL to determine its usefulness in differential diagnosis from MCL. We analyzed the expression of cyclin D1 nuclear protein in paraffin sections using an immunoperoxidase technique in 17 cases diagnosed as SMZL. In eight cases, the presence of cyclin D1 mRNA was also assessed by Northern blot analysis. None of the 17 cases of typical SMZL showed expression of cyclin D1 mRNA or protein. In contrast, four cases used as controls showed either expression of cyclin D1 protein or mRNA or both. We conclude that assessment of cyclin D1 protein expression can be useful in distinguishing SMZL from MCL involving the spleen.

Original languageEnglish (US)
Pages (from-to)601-606
Number of pages6
JournalModern Pathology
Volume11
Issue number7
StatePublished - 1998
Externally publishedYes

Fingerprint

Cyclin D1
Lymphoma
Proteins
bcl-1 Genes
Messenger RNA
Differential Diagnosis
Spleen
Lymphocytes
Mantle-Cell Lymphoma
Lymphocytosis
Nuclear Proteins
Immunoenzyme Techniques
Northern Blotting
Paraffin
Non-Hodgkin's Lymphoma
Chromosomes

Keywords

  • Chronic lymphocytic leukemia
  • Cyclin D1
  • Immunophenotype
  • Mantle cell lymphoma
  • Splenic lymphoma with villous lymphocytes
  • Splenic marginal zone lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Savilo, E., Campo, E., Mollejo, M., Pinyol, M., Piris, M. A., Zukerberg, L. R., ... Harris, N. L. (1998). Absence of cyclin D1 protein expression in splenic marginal zone lymphoma. Modern Pathology, 11(7), 601-606.

Absence of cyclin D1 protein expression in splenic marginal zone lymphoma. / Savilo, Elena; Campo, E.; Mollejo, M.; Pinyol, M.; Piris, M. A.; Zukerberg, L. R.; Yang, W. I.; Koelliker, D. D.; Nguyen, P. L.; Harris, N. L.

In: Modern Pathology, Vol. 11, No. 7, 1998, p. 601-606.

Research output: Contribution to journalArticle

Savilo, E, Campo, E, Mollejo, M, Pinyol, M, Piris, MA, Zukerberg, LR, Yang, WI, Koelliker, DD, Nguyen, PL & Harris, NL 1998, 'Absence of cyclin D1 protein expression in splenic marginal zone lymphoma', Modern Pathology, vol. 11, no. 7, pp. 601-606.
Savilo E, Campo E, Mollejo M, Pinyol M, Piris MA, Zukerberg LR et al. Absence of cyclin D1 protein expression in splenic marginal zone lymphoma. Modern Pathology. 1998;11(7):601-606.
Savilo, Elena ; Campo, E. ; Mollejo, M. ; Pinyol, M. ; Piris, M. A. ; Zukerberg, L. R. ; Yang, W. I. ; Koelliker, D. D. ; Nguyen, P. L. ; Harris, N. L. / Absence of cyclin D1 protein expression in splenic marginal zone lymphoma. In: Modern Pathology. 1998 ; Vol. 11, No. 7. pp. 601-606.
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AU - Zukerberg, L. R.

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AU - Nguyen, P. L.

AU - Harris, N. L.

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