ABP1–TMK auxin perception for global phosphorylation and auxin canalization

Jiří Friml, Michelle Gallei, Zuzana Gelová, Alexander Johnson, Ewa Mazur, Aline Monzer, Lesia Rodriguez, Mark Roosjen, Inge Verstraeten, Branka D. Živanović, Minxia Zou, Lukáš Fiedler, Caterina Giannini, Peter Grones, Mónika Hrtyan, Walter A. Kaufmann, Andre Kuhn, Madhumitha Narasimhan, Marek Randuch, Nikola RýdzaKoji Takahashi, Shutang Tan, Anastasia Teplova, Toshinori Kinoshita, Dolf Weijers, Hana Rakusová

Research output: Contribution to journalArticlepeer-review

Abstract

The phytohormone auxin triggers transcriptional reprogramming through a well-characterized perception machinery in the nucleus. By contrast, mechanisms that underlie fast effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation of proteins or auxin feedback on its transport, remain unclear1–3. Whether auxin-binding protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required for the auxin-induced ultrafast global phospho-response and for downstream processes that include the activation of H+-ATPase and accelerated cytoplasmic streaming. abp1 and tmk mutants cannot establish auxin-transporting channels and show defective auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that lacks the capacity to bind auxin is unable to complement these defects in abp1 mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface signalling, which mediates the global phospho-response and auxin canalization.

Original languageEnglish (US)
JournalNature
DOIs
StatePublished - Sep 15 2022

ASJC Scopus subject areas

  • General

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