Abstract
Myelodysplastic Syndrome (MDS) cells present genetic instability and dysregulation of the gene C3ORF9, which was isolated from an MDS cDNA library and codes for a putative protein. We studied the expression of C3ORF9 in MDS syndromes to contribute to the understanding of the pathophysiology of MDS. One hundred and thirty-one patients and 35 healthy controls were involved in our study. Bone marrow aspirates and isolated CD34+ cells were used. Gene expression was estimated by quantitative PCR. C3ORF9 was found to be down-regulated in patients with CMML compared to the controls (p<0.01). There was no difference between RARS and the controls (p=0.1), while increased expression was found in RA, RAEB and RAEB-T (p<0.01 for all). No mutations or polymorphism were detected in our population. CD34+ cells expressed higher levels of C3ORF9 (p<0.01) in patients. The gene expression was correlated to the percentage of + cells in RAEB and RAEB-T (r=0.64). The altered C3ORF9 expression was possibly due to different gene regulation in these patients and/or to the increased CD34+ cells.
Original language | English (US) |
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Pages (from-to) | 787-793 |
Number of pages | 7 |
Journal | International Journal of Immunopathology and Pharmacology |
Volume | 22 |
Issue number | 3 |
DOIs | |
State | Published - 2009 |
Keywords
- C3ORF9
- Chromosome abnormalities
- MDS
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Pharmacology