A YAC-, P1-, and cosmid-based physical Map of the BRCA1 region on chromosome 17q21

Fergus J. Couch, Lucio H. Castilla, Junzhe Xu, Kenneth J. Abel, Piri Welcsh, Stephanie E. King, Linghua Wong, Peggy P. Ho, Sofia Merajver, Lawrence C. Brody, Guiying Yin, Steve T. Hayes, Linn M. Gieser, Wendy L. Flejter, Thomas W. Glover, Lori S. Friedman, Eric D. Lynch, Jose E. Meza, Mary Claire King, David J. LawLarry Deaven, Anne M. Bowcock, Francis S. Collins, Barbara L. Weber, Settara C. Chandrasekharappa

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

A familial early-onset breast cancer gene (BRCA1) has been localized to chromosome 17q21. To characterize this region and to aid in the identification of the BRCA1 gene, a physical map of a region of 1.0-1.5 Mb between the EDH17B1 and the PPY loci on chromosome 17q21 was generated. The physical map is composed of a yeast artificial chromosome (YAC) and P1 phage contig with one gap. The majority of the interval has also been converted to a cosmid contig. Twenty-three PCR-based sequence-tagged sites (STSs) were mapped to these contigs, thereby confirming the order and overlap of individual clones. This complex physical map of the BRCA1 region was used to isolate genes by a number of gene identification techniques and to generate transcript maps of the region, as presented in the three accompanying manuscripts of Brody et al. (1995), Osborne-Lawrence et al. (1995), and Friedman et al. (1995).

Original languageEnglish (US)
Pages (from-to)264-273
Number of pages10
JournalGenomics
Volume25
Issue number1
DOIs
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Genetics

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    Couch, F. J., Castilla, L. H., Xu, J., Abel, K. J., Welcsh, P., King, S. E., Wong, L., Ho, P. P., Merajver, S., Brody, L. C., Yin, G., Hayes, S. T., Gieser, L. M., Flejter, W. L., Glover, T. W., Friedman, L. S., Lynch, E. D., Meza, J. E., King, M. C., ... Chandrasekharappa, S. C. (1995). A YAC-, P1-, and cosmid-based physical Map of the BRCA1 region on chromosome 17q21. Genomics, 25(1), 264-273. https://doi.org/10.1016/0888-7543(95)80134-8