A unique role for 6-O sulfation modification in zebrafish vascular development

Eleanor Chen, Sally E. Stringer, Melissa A. Rusch, Scott B. Selleck, Stephen C Ekker

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Heparan sulfate proteoglycans are important modulators of growth factor signaling in a variety of patterning processes. Secreted growth factors that play critical roles in angiogenesis bind to heparan sulfate, and this association is affected by 6-O-sulfation of the heparan sulfate chains. Addition of 6-O-sulfate is catalyzed by a family of sulfotransferases (HS6STs), and genetic manipulation of their function permits an assessment of their contribution to vascular assembly. We report on the biochemical activity and expression patterns of two zebrafish HS6ST genes. In situ hybridization reveals dynamic and distinct expression patterns of these two genes during development. Structural analysis of heparan sulfate from wild-type and morpholino antisense 'knockdown' embryos suggests that HS6ST-1 and HS6ST-2 have similar biochemical activity. HS6ST-2, but not HS6ST-1, morphants exhibit abnormalities in the branching morphogenesis of the caudal vein during embryonic development of the zebrafish. Our finding that HS6ST-2 is required for the branching morphogenesis of the caudal vein is the first in vivo evidence for an essential role of a gene encoding a heparan sulfate modifying enzyme in vertebrate angiogenesis. Our analysis of two zebrafish HS6ST genes suggests that a wide range of biological processes may be regulated by an array of sulfation-modifying enzymes in the vertebrate genome.

Original languageEnglish (US)
Pages (from-to)364-376
Number of pages13
JournalDevelopmental Biology
Volume284
Issue number2
DOIs
StatePublished - Aug 15 2005
Externally publishedYes

Fingerprint

Heparitin Sulfate
Zebrafish
Blood Vessels
Morphogenesis
Genes
Vertebrates
Veins
Intercellular Signaling Peptides and Proteins
Biological Phenomena
Sulfotransferases
Morpholinos
Heparan Sulfate Proteoglycans
Enzymes
Sulfates
Embryonic Development
In Situ Hybridization
Embryonic Structures
Genome

Keywords

  • Heparan sulfate 6-O sulfotransferase
  • Morpholino
  • Vascular development
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology

Cite this

A unique role for 6-O sulfation modification in zebrafish vascular development. / Chen, Eleanor; Stringer, Sally E.; Rusch, Melissa A.; Selleck, Scott B.; Ekker, Stephen C.

In: Developmental Biology, Vol. 284, No. 2, 15.08.2005, p. 364-376.

Research output: Contribution to journalArticle

Chen, Eleanor ; Stringer, Sally E. ; Rusch, Melissa A. ; Selleck, Scott B. ; Ekker, Stephen C. / A unique role for 6-O sulfation modification in zebrafish vascular development. In: Developmental Biology. 2005 ; Vol. 284, No. 2. pp. 364-376.
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