A two-center international evaluation of the Immulite 2000 automated serum gastrin assay

Mary L. Eastvold, Hans W. Wallinder, Carol M. Preissner, Ravinder J. Singh, Christian Löwbeer, Stefan K.G. Grebe

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Objectives: To assess the performance of the Immulite 2000 serum gastrin assay. Design and methods: Two-center validation and comparison with two manual gastrin assays. Results: Serum, serum separator, EDTA and heparin tube results differed <20%. Samples showed <20% degradation for <6 h at room temperature, 3-day-refrigerated, and underwent 3 freeze-thaw cycles. Imprecision was <8% between 20 and 824 ng/L (10-393 pmol/L). Spike recovery was 88.5-106%, and recovery on dilution was 62-135%. There were no interferences from gastrin 1-14, pentagastrin, and cholecystokinin (CCK) 1-33 and CCK 26-33. The fasting reference range was <100 ng/L (<48 pmol/L). Regression slopes to the manual assays were 0.9 and 0.97, with comparable clinical performance. Conclusions: The Immulite 2000 assay is precise, accurate, and fast and compares well with established gastrin assays.

Original languageEnglish (US)
Pages (from-to)387-390
Number of pages4
JournalClinical Biochemistry
Volume39
Issue number4
DOIs
StatePublished - Apr 1 2006

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Keywords

  • Automated chemiluminometric immunoassay
  • Gastrin
  • Gastrinoma
  • Pernicious anemia
  • Zollinger-Ellison syndrome

ASJC Scopus subject areas

  • Clinical Biochemistry

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