A Tupaia paramyxovirus vector system for targeting and transgene expression

Christine E. Engeland, Sascha Bossow, Andrew W. Hudacek, Birgit Hoyler, Judith Förster, Rūta Veinalde, Dirk Jäger, Roberto Cattaneo, Guy Ungerechts, Christoph Springfeld

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4 Scopus citations

Abstract

Viruses from the diverse family of Paramyxoviridae include important pathogens and are applied in gene therapy and for cancer treatment. The Tupaia paramyxovirus (TPMV), isolated from the kidney of a tree shrew, does not infect human cells and neutralizing antibodies against other Paramyxoviridae do not cross-react with TPMV. Here, we present a vector system for de novo generation of infectious TPMV that allows for insertion of additional genes as well as targeting using antibody single-chain variable fragments. We show that the recombinant TPMV specifically infect cells expressing the targeted receptor and replicate in human cells. This vector system provides a valuable tool for both basic research and therapeutic applications.

Original languageEnglish (US)
Article number000887
Pages (from-to)2248-2257
Number of pages10
JournalJournal of General Virology
Volume98
Issue number9
DOIs
StatePublished - Sep 2017

Keywords

  • Cellular targeting
  • Gene transfer
  • Paramyxovirus
  • Reverse genetics
  • Tropism
  • Virotherapy

ASJC Scopus subject areas

  • Virology

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    Engeland, C. E., Bossow, S., Hudacek, A. W., Hoyler, B., Förster, J., Veinalde, R., Jäger, D., Cattaneo, R., Ungerechts, G., & Springfeld, C. (2017). A Tupaia paramyxovirus vector system for targeting and transgene expression. Journal of General Virology, 98(9), 2248-2257. [000887]. https://doi.org/10.1099/jgv.0.000887