A transcript map of the chromosome 19q-arm glioma tumor suppressor region

Justin S. Smith, Issei Tachibana, Ute Pohl, Hyun K. Lee, Uma Thanarajasingam, Bryce P. Portier, Keisuke Ueki, Shivapriya Ramaswamy, Stephanie J. Billings, Harvey W. Mohrenweiser, David N. Louis, Robert B. Jenkins

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

Allelic loss of the chromosome 19q arm is a frequent event in human diffuse gliomas, suggesting that it contains a tumor suppressor gene. Recent deletion mapping studies have broadly implicated a 1.6-Mb interval between D19S241E and D19S596, with a limited subset of tumors, suggesting that the region may be as narrow as 150 kb. Focusing on this smaller interval, we have used cDNA selection, exon amplification, and genomic sequencing to identify three novel transcripts (EHD2, GLTSCR1, and GLTSCR2) and to map two known genes (SEPW1 and CRX). A partial transcript map of 19 transcripts and two EST markers has been constructed for the 1.6-Mb interval D19S241E-D19S596. Ten of these transcripts, including the 5 mapped to the 150-kb deletion interval, have been examined for alterations in a panel of gliomas with allelic loss of 19q. Tumor-specific alterations have not been identified in the transcripts examined thus far. Collectively, these data should facilitate subsequent efforts to identify and characterize the remaining transcripts in the 1.6-Mb interval. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)44-50
Number of pages7
JournalGenomics
Volume64
Issue number1
DOIs
StatePublished - Feb 15 2000

ASJC Scopus subject areas

  • Genetics

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    Smith, J. S., Tachibana, I., Pohl, U., Lee, H. K., Thanarajasingam, U., Portier, B. P., Ueki, K., Ramaswamy, S., Billings, S. J., Mohrenweiser, H. W., Louis, D. N., & Jenkins, R. B. (2000). A transcript map of the chromosome 19q-arm glioma tumor suppressor region. Genomics, 64(1), 44-50. https://doi.org/10.1006/geno.1999.6101