A tissue-engineered stent for cell-based vascular gene transfer

Carmelo J. Panetta, Katsumi Miyauchi, David Berry, Robert D. Simari, David R. Holmes, Robert S. Schwartz, Noel M. Caplice

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Cell-based gene transfer using a stent platform would provide a significant advantage in terms of site-specific gene expression in the vasculature. The current study presents a novel stent design that allows stable in vivo transgene expression over a 4-week period in the vasculature. A mesh-stent coated with fibronectin provided an excellent platform for adherent porcine smooth muscle cells (SMC). Autologous porcine SMC were stably transduced with a plasmid encoding green fluorescence protein (GFP), seeded at high density in the mesh-stent, and deployed in the porcine coronary artery. Stable in vivo GFP expression within the mesh-stent (5.2 × 105 GFP-positive cells/cm2 mesh) was demonstrated 1 month after implantation in the porcine coronary artery by fluorescence microscopy and flow cytometry. No significant change in GFP positive cell number within the stent occurred over a 1-month period in vivo when compared to preinsertion. Angiographic and histologic analysis revealed mild neointimal proliferation and no inflammatory infiltrate in the stented segment. This study has implications for treatment of cardiovascular and other diseases where long-term cell-based delivery of transgene is a desirable therapeutic option.

Original languageEnglish (US)
Pages (from-to)433-441
Number of pages9
JournalHuman gene therapy
Volume13
Issue number3
DOIs
StatePublished - Apr 6 2002

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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