A Study on Complicated Chromosome Translocation by Fluorescence in situ Hybridization (FISH) of G-banded Chromosomes

Haojie Huang, Xiran Zhang, Yifeng Chen, Yingxia Cui

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An experimental method of fluorescence in situ hybridization (FISH) with conventionally G-band chromosomes was developed to analysize the complex chromosome rearrangements of patients. In order to obtain optimal results of both G-banding and fluoresence signals, it was essential that G-bandsed chromosomes were fixed with formaldehyde prior to fluorescence in situ hybridization. The karyotype of a patient with spontaneous abortion might be decribed as 46, XX, t(1; 5; 12) (1pter→1q25 : : 12q24→12qter; 5qter→5p11 : : 1q25→1qter; 12pter→12q24 : : 5p11→5pter) by the analysis of conventional cytogenetics. However, her karyotype should be identified as 46, XX, t(1; 5; 12) (1pter→1q23 : : 12q22→12qter; 5qter→5p11 : : 1q25→1qter; 12pter →12q22 : : 1q23→1q25 : : 5p11→5pter) with this newly established method. This investigation indicates that this technique described above is a powerful tool to detect complex chromosome rearrangements of patients.

Original languageEnglish (US)
Pages (from-to)341-342
Number of pages2
JournalActa Genetica Sinica
Volume23
Issue number5
StatePublished - 1996
Externally publishedYes

Fingerprint

Chromosomes, Human, 21-22 and Y
Fluorescence In Situ Hybridization
Chromosomes
Karyotype
Cytogenetic Analysis
Spontaneous Abortion
Formaldehyde

Keywords

  • Chromosome
  • Chromosome G-banding
  • Fluorescence in situ hybridization

ASJC Scopus subject areas

  • Genetics

Cite this

A Study on Complicated Chromosome Translocation by Fluorescence in situ Hybridization (FISH) of G-banded Chromosomes. / Huang, Haojie; Zhang, Xiran; Chen, Yifeng; Cui, Yingxia.

In: Acta Genetica Sinica, Vol. 23, No. 5, 1996, p. 341-342.

Research output: Contribution to journalArticle

@article{fa7746c57c7040a7aa3faac5bcc40050,
title = "A Study on Complicated Chromosome Translocation by Fluorescence in situ Hybridization (FISH) of G-banded Chromosomes",
abstract = "An experimental method of fluorescence in situ hybridization (FISH) with conventionally G-band chromosomes was developed to analysize the complex chromosome rearrangements of patients. In order to obtain optimal results of both G-banding and fluoresence signals, it was essential that G-bandsed chromosomes were fixed with formaldehyde prior to fluorescence in situ hybridization. The karyotype of a patient with spontaneous abortion might be decribed as 46, XX, t(1; 5; 12) (1pter→1q25 : : 12q24→12qter; 5qter→5p11 : : 1q25→1qter; 12pter→12q24 : : 5p11→5pter) by the analysis of conventional cytogenetics. However, her karyotype should be identified as 46, XX, t(1; 5; 12) (1pter→1q23 : : 12q22→12qter; 5qter→5p11 : : 1q25→1qter; 12pter →12q22 : : 1q23→1q25 : : 5p11→5pter) with this newly established method. This investigation indicates that this technique described above is a powerful tool to detect complex chromosome rearrangements of patients.",
keywords = "Chromosome, Chromosome G-banding, Fluorescence in situ hybridization",
author = "Haojie Huang and Xiran Zhang and Yifeng Chen and Yingxia Cui",
year = "1996",
language = "English (US)",
volume = "23",
pages = "341--342",
journal = "Journal of Genetics and Genomics",
issn = "1673-8527",
publisher = "Institute of Genetics and Developmental Biology",
number = "5",

}

TY - JOUR

T1 - A Study on Complicated Chromosome Translocation by Fluorescence in situ Hybridization (FISH) of G-banded Chromosomes

AU - Huang, Haojie

AU - Zhang, Xiran

AU - Chen, Yifeng

AU - Cui, Yingxia

PY - 1996

Y1 - 1996

N2 - An experimental method of fluorescence in situ hybridization (FISH) with conventionally G-band chromosomes was developed to analysize the complex chromosome rearrangements of patients. In order to obtain optimal results of both G-banding and fluoresence signals, it was essential that G-bandsed chromosomes were fixed with formaldehyde prior to fluorescence in situ hybridization. The karyotype of a patient with spontaneous abortion might be decribed as 46, XX, t(1; 5; 12) (1pter→1q25 : : 12q24→12qter; 5qter→5p11 : : 1q25→1qter; 12pter→12q24 : : 5p11→5pter) by the analysis of conventional cytogenetics. However, her karyotype should be identified as 46, XX, t(1; 5; 12) (1pter→1q23 : : 12q22→12qter; 5qter→5p11 : : 1q25→1qter; 12pter →12q22 : : 1q23→1q25 : : 5p11→5pter) with this newly established method. This investigation indicates that this technique described above is a powerful tool to detect complex chromosome rearrangements of patients.

AB - An experimental method of fluorescence in situ hybridization (FISH) with conventionally G-band chromosomes was developed to analysize the complex chromosome rearrangements of patients. In order to obtain optimal results of both G-banding and fluoresence signals, it was essential that G-bandsed chromosomes were fixed with formaldehyde prior to fluorescence in situ hybridization. The karyotype of a patient with spontaneous abortion might be decribed as 46, XX, t(1; 5; 12) (1pter→1q25 : : 12q24→12qter; 5qter→5p11 : : 1q25→1qter; 12pter→12q24 : : 5p11→5pter) by the analysis of conventional cytogenetics. However, her karyotype should be identified as 46, XX, t(1; 5; 12) (1pter→1q23 : : 12q22→12qter; 5qter→5p11 : : 1q25→1qter; 12pter →12q22 : : 1q23→1q25 : : 5p11→5pter) with this newly established method. This investigation indicates that this technique described above is a powerful tool to detect complex chromosome rearrangements of patients.

KW - Chromosome

KW - Chromosome G-banding

KW - Fluorescence in situ hybridization

UR - http://www.scopus.com/inward/record.url?scp=1542741805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542741805&partnerID=8YFLogxK

M3 - Article

C2 - 9041776

AN - SCOPUS:1542741805

VL - 23

SP - 341

EP - 342

JO - Journal of Genetics and Genomics

JF - Journal of Genetics and Genomics

SN - 1673-8527

IS - 5

ER -