A structural model for the large subunit of the mammalian mitochondrial ribosome

Jason A. Mears, Manjuli R. Sharma, Robin R. Gutell, Amanda S. McCook, Paul E. Richardson, Thomas Caulfield, Rajendra K. Agrawal, Stephen C. Harvey

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Protein translation is essential for all forms of life and is conducted by a macromolecular complex, the ribosome. Evolutionary changes in protein and RNA sequences can affect the 3D organization of structural features in ribosomes in different species. The most dramatic changes occur in animal mitochondria, whose genomes have been reduced and altered significantly. The RNA component of the mitochondrial ribosome (mitoribosome) is reduced in size, with a compensatory increase in protein content. Until recently, it was unclear how these changes affect the 3D structure of the mitoribosome. Here, we present a structural model of the large subunit of the mammalian mitoribosome developed by combining molecular modeling techniques with cryo-electron microscopic data at 12.1 Å resolution. The model contains 93% of the mitochondrial rRNA sequence and 16 mitochondrial ribosomal proteins in the large subunit of the mitoribosome. Despite the smaller mitochondrial rRNA, the spatial positions of RNA domains known to be involved directly in protein synthesis are essentially the same as in bacterial and archaeal ribosomes. However, the dramatic reduction in rRNA content necessitates evolution of unique structural features to maintain connectivity between RNA domains. The smaller rRNA sequence also limits the likelihood of tRNA binding at the E-site of the mitoribosome, and correlates with the reduced size of D-loops and T-loops in some animal mitochondrial tRNAs, suggesting co-evolution of mitochondrial rRNA and tRNA structures.

Original languageEnglish (US)
Pages (from-to)193-212
Number of pages20
JournalJournal of Molecular Biology
Volume358
Issue number1
DOIs
StatePublished - Apr 21 2006
Externally publishedYes

Fingerprint

Structural Models
Transfer RNA
Ribosomes
RNA
Macromolecular Substances
Proteins
Ribosomal Proteins
Mitochondrial Proteins
Protein Biosynthesis
Mitochondria
Mitochondrial Ribosomes
Genome
Electrons

Keywords

  • Comparative sequence analysis
  • Mitochondrial evolution
  • Molecular fitting
  • Ribosome
  • rRNA

ASJC Scopus subject areas

  • Virology

Cite this

Mears, J. A., Sharma, M. R., Gutell, R. R., McCook, A. S., Richardson, P. E., Caulfield, T., ... Harvey, S. C. (2006). A structural model for the large subunit of the mammalian mitochondrial ribosome. Journal of Molecular Biology, 358(1), 193-212. https://doi.org/10.1016/j.jmb.2006.01.094

A structural model for the large subunit of the mammalian mitochondrial ribosome. / Mears, Jason A.; Sharma, Manjuli R.; Gutell, Robin R.; McCook, Amanda S.; Richardson, Paul E.; Caulfield, Thomas; Agrawal, Rajendra K.; Harvey, Stephen C.

In: Journal of Molecular Biology, Vol. 358, No. 1, 21.04.2006, p. 193-212.

Research output: Contribution to journalArticle

Mears, JA, Sharma, MR, Gutell, RR, McCook, AS, Richardson, PE, Caulfield, T, Agrawal, RK & Harvey, SC 2006, 'A structural model for the large subunit of the mammalian mitochondrial ribosome', Journal of Molecular Biology, vol. 358, no. 1, pp. 193-212. https://doi.org/10.1016/j.jmb.2006.01.094
Mears, Jason A. ; Sharma, Manjuli R. ; Gutell, Robin R. ; McCook, Amanda S. ; Richardson, Paul E. ; Caulfield, Thomas ; Agrawal, Rajendra K. ; Harvey, Stephen C. / A structural model for the large subunit of the mammalian mitochondrial ribosome. In: Journal of Molecular Biology. 2006 ; Vol. 358, No. 1. pp. 193-212.
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