A spontaneous mutation of integrin α(IIb)β3 (platelet glycoprotein IIb- IIIa) helps define a ligand binding site

M. L. Bajt, M. H. Ginsberg, A. L. Frelinger, M. C. Berndt, J. C. Loftus

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129 Scopus citations


This work characterizes a mutant integrin α(IIb)β3 (glycoprotein (GP) IIb-IIIa) from a thrombasthenic patient, ET, whose platelets fail to aggregate in response to stimuli. The nature of the defect was defined by the reduced ability of synthetic peptide ligands, corresponding to the carboxyl terminus of the fibrinogen γ chain (γ402-411) and Arg-Gly-Asp (RGD), to increase the binding of the occupancy-dependent anti-LIBS1 antibody to mutant α(IIb)β3 and the reduced binding of mutant α(IIb)β3 to an immobilized RGD peptide. In addition, ET's platelets failed to bind the ligand-mimetic monoclonal anti-α(IIb)β3, PAC1. DNA sequence analysis of amplified ET genomic DNA revealed a single G → A base change which encoded substitution of R214 by Q in mature β3. Introduction of this point mutation into recombinant wild type α(IIb)β3 expressed in Chinese hamster ovary cells reproduced the ET platelet α(IIb)β3 deficits in binding of fibrinogen, mAb PAC1, and synthetic peptide ligands. Furthermore, substitution of R214 by Q in the synthetic peptide containing the sequence of β3(211-222) resulted in decreased ability of this peptide to block fibrinogen binding to purified α(IIb)β3. These findings suggest that substitution of β3 R214 by Q is responsible for the functional defect in α(IIb)β3 and that R214 is proximal to or part of a ligand binding domain in α(IIb)β3.

Original languageEnglish (US)
Pages (from-to)3789-3794
Number of pages6
JournalJournal of Biological Chemistry
Issue number6
StatePublished - 1992

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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