A solid phase extraction method for determination of nicotine in serum and urine by isotope dilution gas chromatography/mass spectrometry with selected ion monitoring

Leland B. Baskin, Rodger W. Anderson, Joel R. Charlson, Richard D. Hurt, George M. Lawson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A rapid method for measuring nicotine concentration in serum and urine is described. Deuterated nicotine is used as an internal standard. Nicotine and deuterated nicotine are extracted using a copolymeric-bonded phase silica column. The extract is analysed by gas chromatography coupled with mass spectrometry (GC/MS) operating in selected ion monitoring mode, The method has a lower limit of detection of approximately 2 μg/L and is linear to at least 2000 μg/L. Within-run percentage coefficients of variation (% CV) are < 4 in both assays over a nicotine concentration range of 10-2000 μg/L. Between-run %CV in the serum assay are 5.4, 5.2, 4.8 and 5.9, respectively, at nicotine concentrations of 10, 15, 25, and 50 μg/L. Between-run %CV in the urine assay are 5.9, 4.5, 2.7 and 5.2, respectively, at nicotine concentrations of 100, 250, 500, and 2000 μg/L. The absolute recovery of nicotine is 61 ± 6% (mean ± SD) over the range of 10-250 μg/L. The assay has been used to measure serum nicotine concentrations and 24-h urinary excretion of nicotine to monitor the extent of replacement in subjects receiving transdermal nicotine therapy for smoking cessation.

Original languageEnglish (US)
Pages (from-to)522-527
Number of pages6
JournalAnnals of Clinical Biochemistry
Volume35
Issue number4
StatePublished - 1998

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Solid Phase Extraction
Nicotine
Isotopes
Gas chromatography
Gas Chromatography-Mass Spectrometry
Dilution
Mass spectrometry
Urine
Ions
Monitoring
Serum
Assays
Smoking Cessation
Silicon Dioxide
Gas Chromatography
Limit of Detection
Mass Spectrometry
Recovery

Keywords

  • Serum nicotine
  • Urine nicotine

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

A solid phase extraction method for determination of nicotine in serum and urine by isotope dilution gas chromatography/mass spectrometry with selected ion monitoring. / Baskin, Leland B.; Anderson, Rodger W.; Charlson, Joel R.; Hurt, Richard D.; Lawson, George M.

In: Annals of Clinical Biochemistry, Vol. 35, No. 4, 1998, p. 522-527.

Research output: Contribution to journalArticle

Baskin, Leland B. ; Anderson, Rodger W. ; Charlson, Joel R. ; Hurt, Richard D. ; Lawson, George M. / A solid phase extraction method for determination of nicotine in serum and urine by isotope dilution gas chromatography/mass spectrometry with selected ion monitoring. In: Annals of Clinical Biochemistry. 1998 ; Vol. 35, No. 4. pp. 522-527.
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