A single injection of kisspeptin-54 temporarily increases luteinizing hormone pulsatility in healthy women

C. N. Jayasena, A. N. Comninos, J. D. Veldhuis, S. Misra, A. Abbara, C. Izzi-Engbeaya, M. Donaldson, M. A. Ghatei, S. R. Bloom, W. S. Dhillo

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Background Kisspeptin is a novel hypothalamic peptide which stimulates endogenous gonadotrophin releasing hormone (GnRH) secretion. A single subcutaneous bolus injection of kisspeptin-54 increases circulating luteinizing hormone (LH) levels in women, but its acute effects on LH pulsatility are not known. Aims To investigate the effects of a single subcutaneous (sc) injection of kisspeptin-54 administration on LH pulsatility in healthy female volunteers. Methods Six healthy female adult volunteers underwent 10-minute blood sampling for serum LH measurement for 8 h during the follicular phase of menstrual cycle. Sc bolus injection of saline or kisspeptin-54 (0·15, 0·30 or 0·60 nmol/kg) was administered 4 h after commencing the study. A previously described, blinded deconvolution method was used to detect LH pulses. Results Mean number of LH pulses was increased significantly following 0·30 and 0·60 nmol/kg kisspeptin-54 when compared with saline (mean increase in number of LH pulses per 4 h, following injection: -0·17 ± 0·54, saline; +2·33 ± 0·56, 0·30 nmol/kg kisspeptin-54, P < 0·05 vs saline; +2·33 ± 0·80, 0·60 nmol/kg kisspeptin-54, P < 0·05 vs saline). LH pulse secretory mass increased following injection of 0·60 nmol/kg in five of six subjects, but the mean change in all subjects was non-significant when compared with saline (mean increase in pulse secretory mass in IU/l following injection: +0·35 ± 0·40, saline; +2·61 ± 1·17, 0·60 nmol/kg kisspeptin-54, P = 0·10 vs saline). Conclusions A single injection of kisspeptin-54 temporarily stimulates the number of LH pulses in healthy women. Further studies are required to investigate the therapeutic potential of kisspeptin-54 injection to restore LH pulsatility in patients with reproductive disorders caused by impaired GnRH secretion.

Original languageEnglish (US)
Pages (from-to)558-563
Number of pages6
JournalClinical Endocrinology
Volume79
Issue number4
DOIs
StatePublished - Oct 1 2013

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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    Jayasena, C. N., Comninos, A. N., Veldhuis, J. D., Misra, S., Abbara, A., Izzi-Engbeaya, C., Donaldson, M., Ghatei, M. A., Bloom, S. R., & Dhillo, W. S. (2013). A single injection of kisspeptin-54 temporarily increases luteinizing hormone pulsatility in healthy women. Clinical Endocrinology, 79(4), 558-563. https://doi.org/10.1111/cen.12179