A simple, lanthanide-based method to enhance the transduction efficiency of adenovirus vectors

G. D. Palmer, M. J. Stoddart, E. Gouze, J. N. Gouze, S. C. Ghivizzani, R. M. Porter, Christopher H Evans

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Based upon the powerful bridging and charge-masking properties of lanthanide cations (Ln3+), we have investigated their use to improve the transduction efficiency of adenovirus vectors. Using a luciferase marker gene, it was possible to increase transgene expression by the murine mesenchymal stem cell line C3H10T2-Jan by up to four log orders when using very low multiplicities of infection in conjunction with Ln3+; La3+ was superior to Gd3+, Y3+ and Lu3+ in this regard. All Ln3+ were more effective than Ca2+. Flow cytometry, using a green fluorescent protein marker gene, confirmed that La3+ increased both the percentage of transduced cells and the level of transgene expression per cell. Transduction of primary cultures of a variety of different mesenchymal cells from human, rabbit, bovine and rat sources, as well as gene transfer to synovium and muscle in vivo, was also greatly enhanced. Our findings suggest that this lanthanide-based method holds much promise for expediting both experimental and clinical applications of gene transfer with adenoviral vectors.

Original languageEnglish (US)
Pages (from-to)357-363
Number of pages7
JournalGene Therapy
Volume15
Issue number5
DOIs
StatePublished - Mar 2008
Externally publishedYes

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Lanthanoid Series Elements
Adenoviridae
Transgenes
Genes
Synovial Membrane
Green Fluorescent Proteins
Luciferases
Mesenchymal Stromal Cells
Cations
Flow Cytometry
Rabbits
Cell Line
Muscles
Infection

ASJC Scopus subject areas

  • Genetics

Cite this

Palmer, G. D., Stoddart, M. J., Gouze, E., Gouze, J. N., Ghivizzani, S. C., Porter, R. M., & Evans, C. H. (2008). A simple, lanthanide-based method to enhance the transduction efficiency of adenovirus vectors. Gene Therapy, 15(5), 357-363. https://doi.org/10.1038/sj.gt.3303092

A simple, lanthanide-based method to enhance the transduction efficiency of adenovirus vectors. / Palmer, G. D.; Stoddart, M. J.; Gouze, E.; Gouze, J. N.; Ghivizzani, S. C.; Porter, R. M.; Evans, Christopher H.

In: Gene Therapy, Vol. 15, No. 5, 03.2008, p. 357-363.

Research output: Contribution to journalArticle

Palmer, GD, Stoddart, MJ, Gouze, E, Gouze, JN, Ghivizzani, SC, Porter, RM & Evans, CH 2008, 'A simple, lanthanide-based method to enhance the transduction efficiency of adenovirus vectors', Gene Therapy, vol. 15, no. 5, pp. 357-363. https://doi.org/10.1038/sj.gt.3303092
Palmer GD, Stoddart MJ, Gouze E, Gouze JN, Ghivizzani SC, Porter RM et al. A simple, lanthanide-based method to enhance the transduction efficiency of adenovirus vectors. Gene Therapy. 2008 Mar;15(5):357-363. https://doi.org/10.1038/sj.gt.3303092
Palmer, G. D. ; Stoddart, M. J. ; Gouze, E. ; Gouze, J. N. ; Ghivizzani, S. C. ; Porter, R. M. ; Evans, Christopher H. / A simple, lanthanide-based method to enhance the transduction efficiency of adenovirus vectors. In: Gene Therapy. 2008 ; Vol. 15, No. 5. pp. 357-363.
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