Abstract
A number of software analysis packages for the design of PCR primers are available for PCs; however, software for users that depend on VAX/VMS(TM) operating systems is not available. By treating oligonucleotides as RNA molecules, I have designed an alternative means toward studying oligonucleotide interactions using software that is currently available from The Genetics Computer Group (GCG, Madison, WI). The oligonucleotide interactions with self and non-self are analyzed by the GCG FOLD program, a program which finds a secondary structure of minimum free energy for an RNA molecule. This approach allows the identification of self-priming primer pairs, and the interaction energies provide a guideline for the prediction of optimal PCR primers.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 914-918 |
| Number of pages | 5 |
| Journal | BioTechniques |
| Volume | 13 |
| Issue number | 6 |
| State | Published - 1992 |
ASJC Scopus subject areas
- Biotechnology
- General Biochemistry, Genetics and Molecular Biology