A role for somatomedin C as A differentiating hormone and amplifier of hormone action on ovarian cells: Studies with synthetically pure human somatomedin C and swine granulosa cells

Johannes D. Veldhuis, Lawrence M. Demers

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Isolated swine granulosa cells incubated in chemically defined medium in vitro responded to synthetically pure human somatomedin C IGF-I in a dose and time-dependent fashion with increased pregnenolone, progesterone and estradiol biosynthesis. These stimulatory actions were not mimicked by growth hormone, proinsulin, desoctapeptide insulin, epidermal growth factor, or fibroblast growth factor. Moreover, somatomedin C IGF-I augmented the steroidogenic response of granulosa cells to exogenously supplied sterol substrate in the form of low-density lipoprotein, and amplified the stimulatory actions of the classical ovarian effector hormones, estradiol and follicle-stimulating hormone, in a synergistic fashion. The ability of somatomedin C IGF-I to stimulate estradiol production on the one hand, and to act synergistically with estradiol to stimulate progesterone biosynthesis on the other hand, suggests a unique intrafollicular mechanism for amplifying progestin biosynthetic capacity in granulosa cells.

Original languageEnglish (US)
Pages (from-to)234-240
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume130
Issue number1
DOIs
StatePublished - Jul 16 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'A role for somatomedin C as A differentiating hormone and amplifier of hormone action on ovarian cells: Studies with synthetically pure human somatomedin C and swine granulosa cells'. Together they form a unique fingerprint.

  • Cite this