A role for somatomedin C as A differentiating hormone and amplifier of hormone action on ovarian cells: Studies with synthetically pure human somatomedin C and swine granulosa cells

Johannes D Veldhuis, Lawrence M. Demers

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Isolated swine granulosa cells incubated in chemically defined medium in vitro responded to synthetically pure human somatomedin C IGF-I in a dose and time-dependent fashion with increased pregnenolone, progesterone and estradiol biosynthesis. These stimulatory actions were not mimicked by growth hormone, proinsulin, desoctapeptide insulin, epidermal growth factor, or fibroblast growth factor. Moreover, somatomedin C IGF-I augmented the steroidogenic response of granulosa cells to exogenously supplied sterol substrate in the form of low-density lipoprotein, and amplified the stimulatory actions of the classical ovarian effector hormones, estradiol and follicle-stimulating hormone, in a synergistic fashion. The ability of somatomedin C IGF-I to stimulate estradiol production on the one hand, and to act synergistically with estradiol to stimulate progesterone biosynthesis on the other hand, suggests a unique intrafollicular mechanism for amplifying progestin biosynthetic capacity in granulosa cells.

Original languageEnglish (US)
Pages (from-to)234-240
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume130
Issue number1
DOIs
StatePublished - Jul 16 1985
Externally publishedYes

Fingerprint

Granulosa Cells
Insulin-Like Growth Factor I
Swine
Hormones
Estradiol
Biosynthesis
Progesterone
Proinsulin
Pregnenolone
Fibroblast Growth Factors
Follicle Stimulating Hormone
Sterols
Progestins
LDL Lipoproteins
Epidermal Growth Factor
Growth Hormone
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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abstract = "Isolated swine granulosa cells incubated in chemically defined medium in vitro responded to synthetically pure human somatomedin C IGF-I in a dose and time-dependent fashion with increased pregnenolone, progesterone and estradiol biosynthesis. These stimulatory actions were not mimicked by growth hormone, proinsulin, desoctapeptide insulin, epidermal growth factor, or fibroblast growth factor. Moreover, somatomedin C IGF-I augmented the steroidogenic response of granulosa cells to exogenously supplied sterol substrate in the form of low-density lipoprotein, and amplified the stimulatory actions of the classical ovarian effector hormones, estradiol and follicle-stimulating hormone, in a synergistic fashion. The ability of somatomedin C IGF-I to stimulate estradiol production on the one hand, and to act synergistically with estradiol to stimulate progesterone biosynthesis on the other hand, suggests a unique intrafollicular mechanism for amplifying progestin biosynthetic capacity in granulosa cells.",
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