A role for LEDGF/p75 in targeting HIV DNA integration

Angela Ciuffi, Manuel Llano, Eric Poeschla, Christian Hoffmann, Jeremy Leipzig, Paul Shinn, Joseph R. Ecker, Frederic Bushman

Research output: Contribution to journalArticle

408 Citations (Scopus)

Abstract

HIV DNA integration is favored in active genes, but the underlying mechanism is unclear. Cellular lens epithelium-derived growth factor (LEDGF/p75) binds both chromosomal DNA and HIV integrase, and might therefore direct integration by a tethering interaction. We analyzed HIV integration in cells depleted for LEDGF/p75, and found that integration was (i) less frequent in transcription units, (ii) less frequent in genes regulated by LEDGF/p75 and (iii) more frequent in GC-rich DNA. LEDGF is thus the first example of a cellular protein controlling the location of HIV integration in human cells.

Original languageEnglish (US)
Pages (from-to)1287-1289
Number of pages3
JournalNature Medicine
Volume11
Issue number12
DOIs
StatePublished - Dec 2005

Fingerprint

HIV
DNA
Genes
HIV Integrase
Transcription
Cells
lens epithelium-derived growth factor
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Ciuffi, A., Llano, M., Poeschla, E., Hoffmann, C., Leipzig, J., Shinn, P., ... Bushman, F. (2005). A role for LEDGF/p75 in targeting HIV DNA integration. Nature Medicine, 11(12), 1287-1289. https://doi.org/10.1038/nm1329

A role for LEDGF/p75 in targeting HIV DNA integration. / Ciuffi, Angela; Llano, Manuel; Poeschla, Eric; Hoffmann, Christian; Leipzig, Jeremy; Shinn, Paul; Ecker, Joseph R.; Bushman, Frederic.

In: Nature Medicine, Vol. 11, No. 12, 12.2005, p. 1287-1289.

Research output: Contribution to journalArticle

Ciuffi, A, Llano, M, Poeschla, E, Hoffmann, C, Leipzig, J, Shinn, P, Ecker, JR & Bushman, F 2005, 'A role for LEDGF/p75 in targeting HIV DNA integration', Nature Medicine, vol. 11, no. 12, pp. 1287-1289. https://doi.org/10.1038/nm1329
Ciuffi A, Llano M, Poeschla E, Hoffmann C, Leipzig J, Shinn P et al. A role for LEDGF/p75 in targeting HIV DNA integration. Nature Medicine. 2005 Dec;11(12):1287-1289. https://doi.org/10.1038/nm1329
Ciuffi, Angela ; Llano, Manuel ; Poeschla, Eric ; Hoffmann, Christian ; Leipzig, Jeremy ; Shinn, Paul ; Ecker, Joseph R. ; Bushman, Frederic. / A role for LEDGF/p75 in targeting HIV DNA integration. In: Nature Medicine. 2005 ; Vol. 11, No. 12. pp. 1287-1289.
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