A role for LEDGF/p75 in targeting HIV DNA integration

Angela Ciuffi, Manuel Llano, Eric Poeschla, Christian Hoffmann, Jeremy Leipzig, Paul Shinn, Joseph R. Ecker, Frederic Bushman

Research output: Contribution to journalArticle

429 Scopus citations

Abstract

HIV DNA integration is favored in active genes, but the underlying mechanism is unclear. Cellular lens epithelium-derived growth factor (LEDGF/p75) binds both chromosomal DNA and HIV integrase, and might therefore direct integration by a tethering interaction. We analyzed HIV integration in cells depleted for LEDGF/p75, and found that integration was (i) less frequent in transcription units, (ii) less frequent in genes regulated by LEDGF/p75 and (iii) more frequent in GC-rich DNA. LEDGF is thus the first example of a cellular protein controlling the location of HIV integration in human cells.

Original languageEnglish (US)
Pages (from-to)1287-1289
Number of pages3
JournalNature Medicine
Volume11
Issue number12
DOIs
StatePublished - Dec 1 2005

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Ciuffi, A., Llano, M., Poeschla, E., Hoffmann, C., Leipzig, J., Shinn, P., Ecker, J. R., & Bushman, F. (2005). A role for LEDGF/p75 in targeting HIV DNA integration. Nature Medicine, 11(12), 1287-1289. https://doi.org/10.1038/nm1329