A role for high density lipoproteins in hepatic phosphatidylcholine homeostasis

Zhaoyu Li, Luis B. Agellon, Dennis E. Vance

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Choline is (95%) found largely in the biosphere as a component of phosphatidylcholine (PC) which is made from choline via the CDP-choline pathway. Animals obtain choline from both the diet and via endogenous biosynthesis that involves the conversion of phosphatidylethanolamine into PC by phosphatidylethanolamine N-methyltransferase (PEMT), followed by PC catabolism. We have uncovered a striking gender-specific conservation of choline in female mice that does not occur in male mice. Female Pemt-/- mice maintained hepatic PC/total choline levels during the first day of choline deprivation and escaped liver damage whereas male Pemt-/- mice did not. Plasma PC levels in high-density lipoproteins (HDLs) were higher in male Pemt-/- mice than those in females before choline deprivation. Interestingly, after choline deprivation for 1 day, female, but not male, Pemt-/- mice increased HDL-PC levels. Glybenclamide, an inhibitor of PC efflux mediated by ABC transporters, eliminated this response to choline deprivation in females. These data suggest that (i) increased PC efflux from extra-hepatic tissues to HDLs in the circulation provided sufficient choline for the liver and compensated for loss of hepatic PC during the initial stages of choline deprivation in female, but not male, Pemt-/- mice, and (ii) plasma HDL in female mice has an important function in maintenance of hepatic PC as an acute response to severe choline deprivation.

Original languageEnglish (US)
Pages (from-to)893-900
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1771
Issue number7
DOIs
StatePublished - Jul 2007
Externally publishedYes

Fingerprint

HDL Lipoproteins
Choline
Phosphatidylcholines
Homeostasis
Liver
Phosphatidylethanolamine N-Methyltransferase
Cytidine Diphosphate Choline
ATP-Binding Cassette Transporters
Glyburide
Maintenance
Diet

Keywords

  • Choline
  • Choline deficiency
  • Glybenclamide
  • High density lipoprotein
  • Phosphatidylcholine
  • Phosphatidylethanolamine N-methyltransferase

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

Cite this

A role for high density lipoproteins in hepatic phosphatidylcholine homeostasis. / Li, Zhaoyu; Agellon, Luis B.; Vance, Dennis E.

In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Vol. 1771, No. 7, 07.2007, p. 893-900.

Research output: Contribution to journalArticle

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