A report on the international transglutaminase autoantibody workshop for celiac disease

Marcella Li, Liping Yu, Claudio Tiberti, Margherita Bonamico, Iman Taki, Dongmei Miao, Joseph A Murray, Marian J. Rewers, Edward J. Hoffenberg, Daniel Agardh, Patricia Mueller, Martin Stern, Ezio Bonifacio, Edwin Liu

Research output: Contribution to journalArticle

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Abstract

OBJECTIVES: Measurement of transglutaminase autoantibodies (TGAA) is considered to be the most efficient single serologic test for celiac disease (CD) by the American Gastroenterological Association Institute. We hypothesized that a large international collaborative effort toward improving and standardizing TGAA measurement is both feasible and necessary. The primary aim of this workshop is to compare TGAA assays among various research and clinical laboratories to examine assay concordance and improve (and eventually standardize) the TGAA assay. METHODS: A total of 20 laboratories (5 commercial laboratories, 15 research and clinical laboratories) participated that included enzyme-linked immunosorbent assay (ELISA) and radiobinding assays. A total of 150 serum samples were distributed to each laboratory, with each laboratory receiving an equal aliquot that was coded and blinded, composed of 100 healthy control sera and 50 CD sera. RESULTS: Laboratory sensitivity ranged from 69 to 93 and specificity ranged from 96 to 100. By receiver operator characteristic analysis, the area under the curve (C index) ranged from 0.9488 to 0.9904. When analyzing for linear correlation, r-squared was as high as 0.8882 but as low as 0.4244 for the celiac samples between different laboratories performing ELISA. CONCLUSIONS: This transglutaminase autoantibody workshop allows for larger-scale international participation for the purposes of improving and eventually standardizing the TGAA assay with subsequent workshops.

Original languageEnglish (US)
Pages (from-to)154-163
Number of pages10
JournalAmerican Journal of Gastroenterology
Volume104
Issue number1
DOIs
StatePublished - Jan 2009

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Transglutaminases
Celiac Disease
Autoantibodies
Education
Serum
Enzyme-Linked Immunosorbent Assay
Serologic Tests
Research
Abdomen
Area Under Curve

ASJC Scopus subject areas

  • Gastroenterology

Cite this

A report on the international transglutaminase autoantibody workshop for celiac disease. / Li, Marcella; Yu, Liping; Tiberti, Claudio; Bonamico, Margherita; Taki, Iman; Miao, Dongmei; Murray, Joseph A; Rewers, Marian J.; Hoffenberg, Edward J.; Agardh, Daniel; Mueller, Patricia; Stern, Martin; Bonifacio, Ezio; Liu, Edwin.

In: American Journal of Gastroenterology, Vol. 104, No. 1, 01.2009, p. 154-163.

Research output: Contribution to journalArticle

Li, M, Yu, L, Tiberti, C, Bonamico, M, Taki, I, Miao, D, Murray, JA, Rewers, MJ, Hoffenberg, EJ, Agardh, D, Mueller, P, Stern, M, Bonifacio, E & Liu, E 2009, 'A report on the international transglutaminase autoantibody workshop for celiac disease', American Journal of Gastroenterology, vol. 104, no. 1, pp. 154-163. https://doi.org/10.1038/ajg.2008.8
Li, Marcella ; Yu, Liping ; Tiberti, Claudio ; Bonamico, Margherita ; Taki, Iman ; Miao, Dongmei ; Murray, Joseph A ; Rewers, Marian J. ; Hoffenberg, Edward J. ; Agardh, Daniel ; Mueller, Patricia ; Stern, Martin ; Bonifacio, Ezio ; Liu, Edwin. / A report on the international transglutaminase autoantibody workshop for celiac disease. In: American Journal of Gastroenterology. 2009 ; Vol. 104, No. 1. pp. 154-163.
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