A Replicating Single-Cycle Adenovirus Vaccine Against Ebola Virus

Stephanie S. Anguiano-Zarate, William E. Matchett, Pramod N. Nehete, Jagannadha K. Sastry, Andrea Marzi, Michael A Barry

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Recent West African Ebola virus (EBOV) epidemics have led to testing different anti-EBOV vaccines, including a replication-defective adenovirus (RD-Ad) vector (ChAd3-EBOV) and an infectious, replication-competent recombinant vesicular stomatitis virus expressing the EBOV glycoprotein (rVSV-EBOV; also known as rVSV-ZEBOV). While RD-Ads elicit protection, when scaled up to human trials, the level of protection may be much lower than that of vaccines containing viruses that can replicate. Although a replication-competent Ad (RC-Ad) vaccine might generate a level of protection approximating that of rVSV, this infectious vector would also risk causing adenovirus disease. We recently described a "single-cycle" adenovirus (SC-Ad) vector that amplifies antigen genes like RC-Ad, but that avoids the risk of adenovirus infection. Here we have tested an SC-Ad6 vector expressing the glycoprotein (GP) from a 2014 EBOV strain in mice, hamsters, and rhesus macaques. We show that SC-Ad6-EBOV GP induces a high level of serum antibodies in all species and mediates significant protection against pseudo-challenge with rVSV-EBOV expressing luciferase in mice and hamsters. These data suggest that SC-Ad6-EBOV GP may be useful during future EBOV outbreaks.

Original languageEnglish (US)
Pages (from-to)1883-1889
Number of pages7
JournalThe Journal of infectious diseases
Volume218
Issue number12
DOIs
StatePublished - Nov 5 2018

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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  • Cite this

    Anguiano-Zarate, S. S., Matchett, W. E., Nehete, P. N., Sastry, J. K., Marzi, A., & Barry, M. A. (2018). A Replicating Single-Cycle Adenovirus Vaccine Against Ebola Virus. The Journal of infectious diseases, 218(12), 1883-1889. https://doi.org/10.1093/infdis/jiy411