A reappraisal of immunoglobulin variable gene primers and its impact on assessing clonal relationships between PB B cells and BM plasma cells in AL amyloidosis

Nagaaki Katoh, Tanya L. Poshusta, Michelle K. Manske, Angela Dispenzieri, Morie A. Gertz, Roshini S. Abraham, Marina Ramirez-Alvarado

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Monoclonal tumor plasma cells as well as nonterminally differentiated B cells having a clonal relationship to the tumor cells have been detected in the peripheral blood (PB) of some multiple myeloma (MM) patients but rarely in light chain (primary systemic) amyloidosis (AL) patients. Previously, our group found these peripheral clonotypic B cells in three AL patients. Here, we report detailed analysis of a larger cohort of AL patients to validate the prior findings and to investigate the effect of this cell population on clinical outcome. Fourteen AL patients were selected from a clinical prospective trial, and the relationship between immunoglobulin light chain variable gene (V L) representation in PB B cells and the clonal population in the bone marrow (BM) was investigated. A clonal relationship was not detected, and the present study provides important insights into the disparity with the earlier data, including clinical history of the patients and methodological analysis.

Original languageEnglish (US)
Pages (from-to)1029-1037
Number of pages9
JournalJournal of Clinical Immunology
Volume31
Issue number6
DOIs
StatePublished - Dec 1 2011

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Keywords

  • B cells
  • Clonal relationship
  • Immunoglobulin variable gene primers
  • Light chain amyloidosis
  • Plasma cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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