A rapid and efficient method for purification of rat B cells for complement-dependent cytotoxicity testing

Peter J. Wettstein

Research output: Contribution to journalArticle

Abstract

The development of an effective technique for the enrichment of rat B cells is described. This protocol is an adaptation of the 'panning' technique employing anti-Ig-coated plastic dishes. Rat B cells are bound directly to anti-rat Ig-coated wells in Terasaki test plates where they are typed by complement-dependent cytotoxicity. This technique enriches class II (Ia-like) antigen-positive B cells, facilitating detection with antisera specific for rat class II alloantigens. The utility of this technique in the cell surface phenotyping of minor lymphocyte subpopulations is discussed.

Original languageEnglish (US)
Pages (from-to)65-71
Number of pages7
JournalJournal of Immunological Methods
Volume54
Issue number1
DOIs
StatePublished - 1982

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Keywords

  • complement
  • peripheral blood lymphocytes
  • phosphate-buffered saline - fetal calf serum
  • rat immunoglobulin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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