TY - JOUR
T1 - A quantitative PCR measurement of messenger RNA expression of IGF-I, IGF-II and IGFBP-5 in human skeletal muscle
AU - Schimke, Jill M.Coenen
AU - Ljungqvist, Olle H.
AU - Sarkar, Gobinda
AU - Conover, Cheryl A.
AU - Nair, K. Sreekumaran
N1 - Funding Information:
The authors wish to thank the Clinical Research Center of the University of Vermont for the plasma insulin and glucose analysis and Carole Berg for the whole blood leucine concentration analysis. This study was supported by R01DK41973 and RR00585, RR109 and Mayo Foundation.
PY - 1999/6
Y1 - 1999/6
N2 - Insulin-like growth factor-I and -II (IGF-I and IGF-II) and their binding proteins are important components in growth promotion and tissue maintenance. We determined the presence of IGF-I, -II, and binding protein 5 (IGFBP-5) gene expression in human skeletal muscle and that mRNA abundance is not altered by nutrients and insulin. In the first protocol, (control) subjects were given water. In the second protocol, half of these subjects drank Polycose (carbohydrate) and the remaining subjects drank equal calories as a mixed meal. Quadriceps muscle biopsies were taken at 10 h. A semi- quantitative polymerase chain reaction was designed to measure gene expression. IGF-I, IGF-II and IGFBP-5 mRNA are present in adult human skeletal muscle; but no significant changes between meal groups were observed for IGF-I, IGF-II or IGFBP-5 mRNA levels, indicating that the expression of these genes are not altered acutely by nutrients and insulin.
AB - Insulin-like growth factor-I and -II (IGF-I and IGF-II) and their binding proteins are important components in growth promotion and tissue maintenance. We determined the presence of IGF-I, -II, and binding protein 5 (IGFBP-5) gene expression in human skeletal muscle and that mRNA abundance is not altered by nutrients and insulin. In the first protocol, (control) subjects were given water. In the second protocol, half of these subjects drank Polycose (carbohydrate) and the remaining subjects drank equal calories as a mixed meal. Quadriceps muscle biopsies were taken at 10 h. A semi- quantitative polymerase chain reaction was designed to measure gene expression. IGF-I, IGF-II and IGFBP-5 mRNA are present in adult human skeletal muscle; but no significant changes between meal groups were observed for IGF-I, IGF-II or IGFBP-5 mRNA levels, indicating that the expression of these genes are not altered acutely by nutrients and insulin.
KW - Binding proteins
KW - Insulin-like growth factor
KW - QPCR
KW - mRNA
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U2 - 10.1054/ghir.1999.0104
DO - 10.1054/ghir.1999.0104
M3 - Article
C2 - 10502454
AN - SCOPUS:0032836117
SN - 1096-6374
VL - 9
SP - 179
EP - 186
JO - Growth Hormone and IGF Research
JF - Growth Hormone and IGF Research
IS - 3
ER -