A proteogenomic approach to map the proteome of an unsequenced pathogen - Leishmania donovani

Harsh Pawar, Nandini A. Sahasrabuddhe, Santosh Renuse, Shivakumar Keerthikumar, Jyoti Sharma, Ghantasala S.Sameer Kumar, Abhilash Venugopal, Nirujogi Raja Sekhar, Dhanashree S. Kelkar, Harshal Nemade, Sweta N. Khobragade, Babylakshmi Muthusamy, Kumaran Kandasamy, H. C. Harsha, Raghothama Chaerkady, Milind S. Patole, Akhilesh Pandey

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Visceral leishmaniasis or kala azar is the most severe form of leishmaniasis and is caused by the protozoan parasite Leishmania donovani. There is no published report on L. donovani genome sequence available till date, although the genome sequences of three related Leishmania species are already available. Thus, we took a proteogenomic approach to identify proteins from two different life stages of L. donovani. From our analysis of the promastigote (insect) and amastigote (human) stages of L. donovani, we identified a total of 22322 unique peptides from a homology-based search against proteins from three Leishmania species. These peptides were assigned to 3711 proteins in L. infantum, 3287 proteins in L. major, and 2433 proteins in L. braziliensis. Of the 3711 L. donovani proteins that were identified, the expression of 1387 proteins was detectable in both life stages of the parasite, while 901 and 1423 proteins were identified only in promastigotes and amastigotes life stages, respectively. In addition, we also identified 13 N-terminally and one C-terminally extended proteins based on the proteomic data search against the six-frame translated genome of the three related Leishmania species. Here, we report results from proteomic profiling of L. donovani, an organism with an unsequenced genome.

Original languageEnglish (US)
Pages (from-to)832-844
Number of pages13
JournalProteomics
Volume12
Issue number6
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

Fingerprint

Leishmania donovani
Pathogens
Proteome
Proteins
Leishmania
Genes
Genome
Visceral Leishmaniasis
Proteomics
Parasites
Proteogenomics
Peptides
Leishmaniasis
Insects

Keywords

  • Comparative proteogenomics
  • Digenic parasite
  • Kinetoplastid
  • Microbiology
  • Orthologs
  • Paralogs

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Pawar, H., Sahasrabuddhe, N. A., Renuse, S., Keerthikumar, S., Sharma, J., Kumar, G. S. S., ... Pandey, A. (2012). A proteogenomic approach to map the proteome of an unsequenced pathogen - Leishmania donovani. Proteomics, 12(6), 832-844. https://doi.org/10.1002/pmic.201100505

A proteogenomic approach to map the proteome of an unsequenced pathogen - Leishmania donovani. / Pawar, Harsh; Sahasrabuddhe, Nandini A.; Renuse, Santosh; Keerthikumar, Shivakumar; Sharma, Jyoti; Kumar, Ghantasala S.Sameer; Venugopal, Abhilash; Sekhar, Nirujogi Raja; Kelkar, Dhanashree S.; Nemade, Harshal; Khobragade, Sweta N.; Muthusamy, Babylakshmi; Kandasamy, Kumaran; Harsha, H. C.; Chaerkady, Raghothama; Patole, Milind S.; Pandey, Akhilesh.

In: Proteomics, Vol. 12, No. 6, 01.03.2012, p. 832-844.

Research output: Contribution to journalArticle

Pawar, H, Sahasrabuddhe, NA, Renuse, S, Keerthikumar, S, Sharma, J, Kumar, GSS, Venugopal, A, Sekhar, NR, Kelkar, DS, Nemade, H, Khobragade, SN, Muthusamy, B, Kandasamy, K, Harsha, HC, Chaerkady, R, Patole, MS & Pandey, A 2012, 'A proteogenomic approach to map the proteome of an unsequenced pathogen - Leishmania donovani', Proteomics, vol. 12, no. 6, pp. 832-844. https://doi.org/10.1002/pmic.201100505
Pawar H, Sahasrabuddhe NA, Renuse S, Keerthikumar S, Sharma J, Kumar GSS et al. A proteogenomic approach to map the proteome of an unsequenced pathogen - Leishmania donovani. Proteomics. 2012 Mar 1;12(6):832-844. https://doi.org/10.1002/pmic.201100505
Pawar, Harsh ; Sahasrabuddhe, Nandini A. ; Renuse, Santosh ; Keerthikumar, Shivakumar ; Sharma, Jyoti ; Kumar, Ghantasala S.Sameer ; Venugopal, Abhilash ; Sekhar, Nirujogi Raja ; Kelkar, Dhanashree S. ; Nemade, Harshal ; Khobragade, Sweta N. ; Muthusamy, Babylakshmi ; Kandasamy, Kumaran ; Harsha, H. C. ; Chaerkady, Raghothama ; Patole, Milind S. ; Pandey, Akhilesh. / A proteogenomic approach to map the proteome of an unsequenced pathogen - Leishmania donovani. In: Proteomics. 2012 ; Vol. 12, No. 6. pp. 832-844.
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