A prospective comparison of digital image analysis and routine cytology for the identification of malignancy in biliary tract strictures

Todd H. Baron, Gavin C. Harewood, Ashwin Rumalla, Nicole L. Pochron, Linda M. Stadheim, Gregory James Gores, Terry M Therneau, Piet C. De Groen, Thomas J. Sebo, Diva R. Salomao, Benjamin R. Kipp

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Background & Aims: Digital image analysis (DIA) allows quantification of nuclear DNA content and may help distinguish benign and malignant strictures of the biliary tract. Methods: One hundred ten consecutive patients undergoing endoscopic retrograde cholangiography for suspicious biliary tract strictures were enrolled in a prospective study comparing the accuracy of DIA and routine cytology (RC). Standard brush cytology sampling was performed twice by using 2 cytology brushes per patient. Both brushes were fixed in a single-specimen vial. Each specimen was formed into 1 pellet, and the sample was equally divided for evaluation by DIA and RC. DNA histograms were generated for ploidy analysis. The DIA criterion for malignancy was demonstration of aneuploidy. Results: Two patients had inadequate samples obtained for DIA analysis, 7 benign patients were excluded because of inadequate follow-up of less than 75 days, and 1 patient was lost to follow-up to clarify malignant versus benign disease. Of the remaining 100 patients, 56 strictures were malignant and 44 were benign. The sensitivities of DIA and RC were 39.3% and 17.9%, respectively (P = 0.014). The specificities of DIA and RC were 77.3% and 97.7%, respectively (P = 0.003). The accuracy of DIA (56.0%) was equivalent to RC (53.0%). Conclusions: DIA is a valuable adjunct to RC for detecting malignant strictures of the biliary tract.

Original languageEnglish (US)
Pages (from-to)214-219
Number of pages6
JournalClinical Gastroenterology and Hepatology
Volume2
Issue number3
DOIs
StatePublished - Mar 2004

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Biliary Tract
Cell Biology
Pathologic Constriction
Neoplasms
Cholangiography
Ploidies
Lost to Follow-Up
DNA
Aneuploidy
Prospective Studies

Keywords

  • DIA
  • Digital image analysis
  • Endoscopic retrograde cholangiopancreatography
  • ERCP
  • PDI
  • Peak DNA indice
  • RC
  • Routine cytology

ASJC Scopus subject areas

  • Gastroenterology

Cite this

A prospective comparison of digital image analysis and routine cytology for the identification of malignancy in biliary tract strictures. / Baron, Todd H.; Harewood, Gavin C.; Rumalla, Ashwin; Pochron, Nicole L.; Stadheim, Linda M.; Gores, Gregory James; Therneau, Terry M; De Groen, Piet C.; Sebo, Thomas J.; Salomao, Diva R.; Kipp, Benjamin R.

In: Clinical Gastroenterology and Hepatology, Vol. 2, No. 3, 03.2004, p. 214-219.

Research output: Contribution to journalArticle

Baron, Todd H. ; Harewood, Gavin C. ; Rumalla, Ashwin ; Pochron, Nicole L. ; Stadheim, Linda M. ; Gores, Gregory James ; Therneau, Terry M ; De Groen, Piet C. ; Sebo, Thomas J. ; Salomao, Diva R. ; Kipp, Benjamin R. / A prospective comparison of digital image analysis and routine cytology for the identification of malignancy in biliary tract strictures. In: Clinical Gastroenterology and Hepatology. 2004 ; Vol. 2, No. 3. pp. 214-219.
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T1 - A prospective comparison of digital image analysis and routine cytology for the identification of malignancy in biliary tract strictures

AU - Baron, Todd H.

AU - Harewood, Gavin C.

AU - Rumalla, Ashwin

AU - Pochron, Nicole L.

AU - Stadheim, Linda M.

AU - Gores, Gregory James

AU - Therneau, Terry M

AU - De Groen, Piet C.

AU - Sebo, Thomas J.

AU - Salomao, Diva R.

AU - Kipp, Benjamin R.

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N2 - Background & Aims: Digital image analysis (DIA) allows quantification of nuclear DNA content and may help distinguish benign and malignant strictures of the biliary tract. Methods: One hundred ten consecutive patients undergoing endoscopic retrograde cholangiography for suspicious biliary tract strictures were enrolled in a prospective study comparing the accuracy of DIA and routine cytology (RC). Standard brush cytology sampling was performed twice by using 2 cytology brushes per patient. Both brushes were fixed in a single-specimen vial. Each specimen was formed into 1 pellet, and the sample was equally divided for evaluation by DIA and RC. DNA histograms were generated for ploidy analysis. The DIA criterion for malignancy was demonstration of aneuploidy. Results: Two patients had inadequate samples obtained for DIA analysis, 7 benign patients were excluded because of inadequate follow-up of less than 75 days, and 1 patient was lost to follow-up to clarify malignant versus benign disease. Of the remaining 100 patients, 56 strictures were malignant and 44 were benign. The sensitivities of DIA and RC were 39.3% and 17.9%, respectively (P = 0.014). The specificities of DIA and RC were 77.3% and 97.7%, respectively (P = 0.003). The accuracy of DIA (56.0%) was equivalent to RC (53.0%). Conclusions: DIA is a valuable adjunct to RC for detecting malignant strictures of the biliary tract.

AB - Background & Aims: Digital image analysis (DIA) allows quantification of nuclear DNA content and may help distinguish benign and malignant strictures of the biliary tract. Methods: One hundred ten consecutive patients undergoing endoscopic retrograde cholangiography for suspicious biliary tract strictures were enrolled in a prospective study comparing the accuracy of DIA and routine cytology (RC). Standard brush cytology sampling was performed twice by using 2 cytology brushes per patient. Both brushes were fixed in a single-specimen vial. Each specimen was formed into 1 pellet, and the sample was equally divided for evaluation by DIA and RC. DNA histograms were generated for ploidy analysis. The DIA criterion for malignancy was demonstration of aneuploidy. Results: Two patients had inadequate samples obtained for DIA analysis, 7 benign patients were excluded because of inadequate follow-up of less than 75 days, and 1 patient was lost to follow-up to clarify malignant versus benign disease. Of the remaining 100 patients, 56 strictures were malignant and 44 were benign. The sensitivities of DIA and RC were 39.3% and 17.9%, respectively (P = 0.014). The specificities of DIA and RC were 77.3% and 97.7%, respectively (P = 0.003). The accuracy of DIA (56.0%) was equivalent to RC (53.0%). Conclusions: DIA is a valuable adjunct to RC for detecting malignant strictures of the biliary tract.

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