A primer for morpholino use in zebrafish

Brent R. Bill, Andrew M. Petzold, Karl J Clark, Lisa A. Schimmenti, Stephen C Ekker

Research output: Contribution to journalArticle

259 Citations (Scopus)

Abstract

Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.

Original languageEnglish (US)
Pages (from-to)69-77
Number of pages9
JournalZebrafish
Volume6
Issue number1
DOIs
StatePublished - Mar 1 2009

Fingerprint

Morpholinos
Zebrafish
Danio rerio
complementary DNA
oligonucleotides
vertebrates
Confounding Factors (Epidemiology)
injection
Vertebrates
methodology
Complementary DNA
Technology
Injections

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

Cite this

A primer for morpholino use in zebrafish. / Bill, Brent R.; Petzold, Andrew M.; Clark, Karl J; Schimmenti, Lisa A.; Ekker, Stephen C.

In: Zebrafish, Vol. 6, No. 1, 01.03.2009, p. 69-77.

Research output: Contribution to journalArticle

Bill, BR, Petzold, AM, Clark, KJ, Schimmenti, LA & Ekker, SC 2009, 'A primer for morpholino use in zebrafish', Zebrafish, vol. 6, no. 1, pp. 69-77. https://doi.org/10.1089/zeb.2008.0555
Bill, Brent R. ; Petzold, Andrew M. ; Clark, Karl J ; Schimmenti, Lisa A. ; Ekker, Stephen C. / A primer for morpholino use in zebrafish. In: Zebrafish. 2009 ; Vol. 6, No. 1. pp. 69-77.
@article{c79bb79fa4774d29b0c89ba619029fff,
title = "A primer for morpholino use in zebrafish",
abstract = "Morpholino oligonucleotides are the most common anti-sense {"}knockdown{"} technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.",
author = "Bill, {Brent R.} and Petzold, {Andrew M.} and Clark, {Karl J} and Schimmenti, {Lisa A.} and Ekker, {Stephen C}",
year = "2009",
month = "3",
day = "1",
doi = "10.1089/zeb.2008.0555",
language = "English (US)",
volume = "6",
pages = "69--77",
journal = "Zebrafish",
issn = "1545-8547",
publisher = "Mary Ann Liebert Inc.",
number = "1",

}

TY - JOUR

T1 - A primer for morpholino use in zebrafish

AU - Bill, Brent R.

AU - Petzold, Andrew M.

AU - Clark, Karl J

AU - Schimmenti, Lisa A.

AU - Ekker, Stephen C

PY - 2009/3/1

Y1 - 2009/3/1

N2 - Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.

AB - Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.

UR - http://www.scopus.com/inward/record.url?scp=65349149376&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65349149376&partnerID=8YFLogxK

U2 - 10.1089/zeb.2008.0555

DO - 10.1089/zeb.2008.0555

M3 - Article

C2 - 19374550

AN - SCOPUS:65349149376

VL - 6

SP - 69

EP - 77

JO - Zebrafish

JF - Zebrafish

SN - 1545-8547

IS - 1

ER -