A primer for morpholino use in zebrafish

Brent R. Bill, Andrew M. Petzold, Karl J. Clark, Lisa A. Schimmenti, Stephen C. Ekker

Research output: Contribution to journalReview articlepeer-review

280 Scopus citations

Abstract

Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.

Original languageEnglish (US)
Pages (from-to)69-77
Number of pages9
JournalZebrafish
Volume6
Issue number1
DOIs
StatePublished - Mar 1 2009

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

Fingerprint

Dive into the research topics of 'A primer for morpholino use in zebrafish'. Together they form a unique fingerprint.

Cite this