TY - JOUR
T1 - A potential H-DNA element in the MUC1 promoter does not influence transcription
AU - Pahwa, Gurcharan S.
AU - James Maher, L.
AU - Hollingsworth, Michael A.
PY - 1996
Y1 - 1996
N2 - A purine/pyrimidine mirror repeat element (M-PMR3) in the MUC1 promoter has been shown to form H-DNA under in vitro conditions. We investigated this element for biological function in the regulation of transcription of this gene. Chloramphenicol acetyltransferase reporter-promoter constructs were prepared in which the mirror repeat element (PMR3) was intact, deleted, or modified, and their activities were evaluated by transient transfection assays into the cell lines Capan-2, PANC1, and HT-29. Deletion or modification of M-PMR3 increased expression of chloramphenicol acetyltransferase activity in MUC1-expressing cells; however, a role for an H-DNA structure in this activity was not supported by the results.
AB - A purine/pyrimidine mirror repeat element (M-PMR3) in the MUC1 promoter has been shown to form H-DNA under in vitro conditions. We investigated this element for biological function in the regulation of transcription of this gene. Chloramphenicol acetyltransferase reporter-promoter constructs were prepared in which the mirror repeat element (PMR3) was intact, deleted, or modified, and their activities were evaluated by transient transfection assays into the cell lines Capan-2, PANC1, and HT-29. Deletion or modification of M-PMR3 increased expression of chloramphenicol acetyltransferase activity in MUC1-expressing cells; however, a role for an H-DNA structure in this activity was not supported by the results.
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U2 - 10.1074/jbc.271.43.26543
DO - 10.1074/jbc.271.43.26543
M3 - Article
C2 - 8900124
AN - SCOPUS:10244257589
SN - 0021-9258
VL - 271
SP - 26543
EP - 26546
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 43
ER -