TY - JOUR
T1 - A one-step method for protein estimation in biological samples
T2 - Nitration of tyrosine in nitric acid
AU - Bible, Keith C.
AU - Boerner, Scott A.
AU - Kaufmann, Scott H.
N1 - Funding Information:
This work was supported in part by grants from the NIH (CA 67818 and CA 69008) and the Jack Taylor Family Foundation. The secretarial assistance of Deb Strauss is gratefully acknowledged.
PY - 1999/2/1
Y1 - 1999/2/1
N2 - A number of methods are commonly employed for the determination of protein in biological samples. Unfortunately, several compounds that are constituents of biological buffers interfere with these methods, limiting their application. Previous studies have demonstrated that tyrosine rapidly undergoes nitration in nitric acid to yield 3-nitrotyrosine, which has a λ(max) of 358 nm. Utilizing this reaction, we have developed a one-step method for the assessment of protein content in biological samples. Common interfering substances, including SDS, urea, glycerol, ammonium sulfate, and β-mercaptoethanol, do not interfere with this method. Because of its simplicity, this reaction might be useful for estimating protein content in a variety of biological samples.
AB - A number of methods are commonly employed for the determination of protein in biological samples. Unfortunately, several compounds that are constituents of biological buffers interfere with these methods, limiting their application. Previous studies have demonstrated that tyrosine rapidly undergoes nitration in nitric acid to yield 3-nitrotyrosine, which has a λ(max) of 358 nm. Utilizing this reaction, we have developed a one-step method for the assessment of protein content in biological samples. Common interfering substances, including SDS, urea, glycerol, ammonium sulfate, and β-mercaptoethanol, do not interfere with this method. Because of its simplicity, this reaction might be useful for estimating protein content in a variety of biological samples.
KW - 3-nitrotyrosine
KW - Nitrotyrosine
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U2 - 10.1006/abio.1998.2980
DO - 10.1006/abio.1998.2980
M3 - Article
C2 - 9918674
AN - SCOPUS:0033080744
SN - 0003-2697
VL - 267
SP - 217
EP - 221
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -