A one-step method for protein estimation in biological samples: Nitration of tyrosine in nitric acid

Keith C. Bible, Scott A. Boerner, Scott H. Kaufmann

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A number of methods are commonly employed for the determination of protein in biological samples. Unfortunately, several compounds that are constituents of biological buffers interfere with these methods, limiting their application. Previous studies have demonstrated that tyrosine rapidly undergoes nitration in nitric acid to yield 3-nitrotyrosine, which has a λ(max) of 358 nm. Utilizing this reaction, we have developed a one-step method for the assessment of protein content in biological samples. Common interfering substances, including SDS, urea, glycerol, ammonium sulfate, and β-mercaptoethanol, do not interfere with this method. Because of its simplicity, this reaction might be useful for estimating protein content in a variety of biological samples.

Original languageEnglish (US)
Pages (from-to)217-221
Number of pages5
JournalAnalytical Biochemistry
Volume267
Issue number1
DOIs
StatePublished - Feb 1 1999

Keywords

  • 3-nitrotyrosine
  • Nitrotyrosine

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'A one-step method for protein estimation in biological samples: Nitration of tyrosine in nitric acid'. Together they form a unique fingerprint.

  • Cite this