A nuclear factor that binds purine-rich, single-stranded oligonucleotides derived from S1-sensitive elements upstream of the CFTR gene and the MUC1 gene

Michael A. Hollingsworth, Christina Closken, Ann Harris, Claudia D. McDonald, Gurcharan S. Pahwa, L James Maher III

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We have identified two regions of non-random purine/pyrimidine strand asymmetry that were nearly identical in sequence in the 5' flanking (promoter) regions of the human cystic fibrosis transmembrane conductance regulator (CFTR) gene and the human MUC1 gene. These regions contain perfect mirror repeat elements, a sequence motif previously found to be associated with the formation of H-DNA conformations. In this report we demonstrate that a single-stranded non-B DNA conformation exists at low pH in supercoiled plasmids containing the similar mirror repeat elements, and that S1 nuclease digestion maps the single-stranded region to the position of the mirror repeats. In addition, we identify a nuclear protein of approximately 27 kD that binds to single-stranded oligonucleotides corresponding to the purine-rich strand of this region, but not to the pyrimidine-rich strands or to double-stranded oligonucleotides with corresponding purine/pyrimidine strand asymmetry.

Original languageEnglish (US)
Pages (from-to)1138-1146
Number of pages9
JournalNucleic Acids Research
Volume22
Issue number7
StatePublished - Apr 11 1994
Externally publishedYes

Fingerprint

Fibrosis
Cystic Fibrosis Transmembrane Conductance Regulator
Oligonucleotides
Regulator Genes
Conductance
Regulator
Nucleic Acid Conformation
Mirrors
Genes
Gene
Conformations
DNA
Mirror
Conformation
5' Flanking Region
Asymmetry
Nuclear Proteins
Genetic Promoter Regions
Digestion
Plasmids

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

A nuclear factor that binds purine-rich, single-stranded oligonucleotides derived from S1-sensitive elements upstream of the CFTR gene and the MUC1 gene. / Hollingsworth, Michael A.; Closken, Christina; Harris, Ann; McDonald, Claudia D.; Pahwa, Gurcharan S.; Maher III, L James.

In: Nucleic Acids Research, Vol. 22, No. 7, 11.04.1994, p. 1138-1146.

Research output: Contribution to journalArticle

Hollingsworth, Michael A. ; Closken, Christina ; Harris, Ann ; McDonald, Claudia D. ; Pahwa, Gurcharan S. ; Maher III, L James. / A nuclear factor that binds purine-rich, single-stranded oligonucleotides derived from S1-sensitive elements upstream of the CFTR gene and the MUC1 gene. In: Nucleic Acids Research. 1994 ; Vol. 22, No. 7. pp. 1138-1146.
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