TY - JOUR
T1 - A nuclear factor that binds purine-rich, single-stranded oligonucleotides derived from S1-sensitive elements upstream of the CFTR gene and the MUC1 gene
AU - Hollingsworth, Michael A.
AU - Closken, Christina
AU - Harris, Ann
AU - Mcdonald, Claudia D.
AU - Pahwa, Gurcharan S.
AU - Maher, L. James
N1 - Funding Information:
The authors would like to thank Thomas Caffrey and John Skoog for technical assistance, and Tracy Culjat for secretarial assistance. This work was supported in part by the following grants: From the National Institutes of Health, DK44762, CA57362, GM47814 and CA36727; from the American Cancer Society, SIG 16; a grant from the Cystic Fibrosis Research Trust, UK; and by a Young Investigator's Award from Abbott Laboratories and a Junior Faculty Research Award from the American Cancer Society (both to L.J.M.)
PY - 1994/4/11
Y1 - 1994/4/11
N2 - We have identified two regions of non-random purine/pyrimldine strand asymmetry that were nearly identical in sequence in the 5′ flanking (promoter) regions of the human cystic fibrosis transmembrane conductance regulator (CFTR) gene and the human MUC1 gene. These regions contain perfect mirror repeat elements, a sequence motif previously found to be associated with the formation of H-DNA conformations. In this report we demonstrate that a single-stranded non-B DNA conformation exists at low pH in supercoiled plasmids containing the similar mirror repeat elements, and that S1 nuclease digestion maps the single-stranded region to the position of the mirror repeats. In addition, we identify a nuclear protein of approximately 27 kD that binds to single-stranded oligonucleotides corresponding to the purtne-rlch strand of this region, but not to the pyrlmldlne-rlch strands or to double-stranded oligonucleotides with corresponding purine/pyrimidlne strand asymmetry.
AB - We have identified two regions of non-random purine/pyrimldine strand asymmetry that were nearly identical in sequence in the 5′ flanking (promoter) regions of the human cystic fibrosis transmembrane conductance regulator (CFTR) gene and the human MUC1 gene. These regions contain perfect mirror repeat elements, a sequence motif previously found to be associated with the formation of H-DNA conformations. In this report we demonstrate that a single-stranded non-B DNA conformation exists at low pH in supercoiled plasmids containing the similar mirror repeat elements, and that S1 nuclease digestion maps the single-stranded region to the position of the mirror repeats. In addition, we identify a nuclear protein of approximately 27 kD that binds to single-stranded oligonucleotides corresponding to the purtne-rlch strand of this region, but not to the pyrlmldlne-rlch strands or to double-stranded oligonucleotides with corresponding purine/pyrimidlne strand asymmetry.
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U2 - 10.1093/nar/22.7.1138
DO - 10.1093/nar/22.7.1138
M3 - Article
C2 - 7513081
AN - SCOPUS:0028240512
SN - 0305-1048
VL - 22
SP - 1138
EP - 1146
JO - Nucleic acids research
JF - Nucleic acids research
IS - 7
ER -