A novel role for p120 catenin in E-cadherin function

Reneé C. Ireton, Michael A. Davis, Jolanda Van Hengel, Deborah J. Mariner, Kirk Barnes, Molly A. Thoreson, Panagiotis Z Anastasiadis, Linsey Matrisian, Linda M. Bundy, Linda Sealy, Barbara Gilbert, Frans Van Roy, Albert B. Reynolds

Research output: Contribution to journalArticle

413 Citations (Scopus)

Abstract

Indirect evidence suggests that p120-catenin (p120) can both positively and negatively affect cadherin adhesiveness. Here we show that the p120 gene is mutated in SW48 cells, and that the cadherin adhesion system is impaired as a direct consequence of p120 insufficiency. Restoring normal levels of p120 caused a striking reversion from poorly differentiated to cobblestone-like epithelial morphology, indicating a crucial role for p120 in reactivation of E-cadherin function. The rescue efficiency was enhanced by increased levels of p120, and reduced by the presence of the phosphorylation domain, a region previously postulated to confer negative regulation. Surprisingly, the rescue was associated with substantially increased levels of E-cadherin. E-cadherin mRNA levels were unaffected by p120 expression, but E-cadherin half-life was more than doubled. Direct p120-E-cadherin interaction was crucial, as p120 deletion analysis revealed a perfect correlation between E-cadherin binding and rescue of epithelial morphology. Interestingly, the epithelial morphology could also be rescued by forced expression of either WT E-cadherin or a p120-uncoupled mutant. Thus, the effects of uncoupling p120 from E-cadherin can be at least partially overcome by artificially maintaining high levels of cadherin expression. These data reveal a cooperative interaction between p120 and E-cadherin and a novel role for p120 that is likely indispensable in normal cells.

Original languageEnglish (US)
Pages (from-to)465-476
Number of pages12
JournalJournal of Cell Biology
Volume159
Issue number3
DOIs
StatePublished - Nov 11 2002
Externally publishedYes

Fingerprint

Cadherins
delta catenin
Adhesiveness
Cell Adhesion
Half-Life
Phosphorylation

Keywords

  • Cadherin
  • Catenin
  • p120
  • p120ctn
  • SW48

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ireton, R. C., Davis, M. A., Van Hengel, J., Mariner, D. J., Barnes, K., Thoreson, M. A., ... Reynolds, A. B. (2002). A novel role for p120 catenin in E-cadherin function. Journal of Cell Biology, 159(3), 465-476. https://doi.org/10.1083/jcb.200205115

A novel role for p120 catenin in E-cadherin function. / Ireton, Reneé C.; Davis, Michael A.; Van Hengel, Jolanda; Mariner, Deborah J.; Barnes, Kirk; Thoreson, Molly A.; Anastasiadis, Panagiotis Z; Matrisian, Linsey; Bundy, Linda M.; Sealy, Linda; Gilbert, Barbara; Van Roy, Frans; Reynolds, Albert B.

In: Journal of Cell Biology, Vol. 159, No. 3, 11.11.2002, p. 465-476.

Research output: Contribution to journalArticle

Ireton, RC, Davis, MA, Van Hengel, J, Mariner, DJ, Barnes, K, Thoreson, MA, Anastasiadis, PZ, Matrisian, L, Bundy, LM, Sealy, L, Gilbert, B, Van Roy, F & Reynolds, AB 2002, 'A novel role for p120 catenin in E-cadherin function', Journal of Cell Biology, vol. 159, no. 3, pp. 465-476. https://doi.org/10.1083/jcb.200205115
Ireton RC, Davis MA, Van Hengel J, Mariner DJ, Barnes K, Thoreson MA et al. A novel role for p120 catenin in E-cadherin function. Journal of Cell Biology. 2002 Nov 11;159(3):465-476. https://doi.org/10.1083/jcb.200205115
Ireton, Reneé C. ; Davis, Michael A. ; Van Hengel, Jolanda ; Mariner, Deborah J. ; Barnes, Kirk ; Thoreson, Molly A. ; Anastasiadis, Panagiotis Z ; Matrisian, Linsey ; Bundy, Linda M. ; Sealy, Linda ; Gilbert, Barbara ; Van Roy, Frans ; Reynolds, Albert B. / A novel role for p120 catenin in E-cadherin function. In: Journal of Cell Biology. 2002 ; Vol. 159, No. 3. pp. 465-476.
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