A novel region of deletion on chromosome 6q23.3 spanning less than 500 Kb in high grade invasive epithelial ovarian cancer

Vijayalakshmi Shridhar, Julie Staub, Brenda Huntley, William Arthur Cliby, Robert Brian Jenkins, Harvey I. Pass, Lynn Hartmann, David I Smith

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Detailed deletion mapping of chromosome 6q sequences in invasive ovarian tumors have implicated several broad regions involving 6q14-16, 6q21-23, 6q25-26, and the telomeric portion in band 6q27 as regions of frequent loss in this malignancy. In order to define regions of loss involved in the development of ovarian cancer, we used 23 polymorphic markers on 6q to examine allelic loss in 25 high-grade, late stage ovarian tumors. Four non-overlapping deletion regions were observed: (1) at 6q21-22.3 (D6S301-D6S292); (2) within a 1 cM region at 23.2-23.3 between markers D6S978-D6S1637 (at D6S311); (3) at 6q26 (between markers D6S411-D6S1277) and (4) at 6q27 with the markers D6S297 and D6S193. The highest region of loss was observed with marker D6S311 (lost in 17 of 19 informative cases, 89%) in 6q23.3, followed by D6S977 and D6S1637 (71 and 55%, respectively). The average fractional allele loss in the high-grade tumors was around 35%. Previous reports have shown 6q27 as the region of most frequent loss in invasive ovarian cancer. However, our results indicate a novel region in 6q23.3 (spanning less than 500 Kb distance between the markers) with the highest loss, implicating this region of chromosome 6q to harbor a putative tumor suppressor gene involved in the development of invasive epithelial ovarian cancer.

Original languageEnglish (US)
Pages (from-to)3913-3918
Number of pages6
JournalOncogene
Volume18
Issue number26
DOIs
StatePublished - Jul 1 1999

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Chromosome Deletion
Ovarian Neoplasms
Neoplasms
Loss of Heterozygosity
Chromosome Mapping
Tumor Suppressor Genes
Chromosomes
Alleles
Ovarian epithelial cancer

Keywords

  • 6q23.3 loss
  • Invasive epithelial ovarian cancer
  • Tumor suppressor

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

A novel region of deletion on chromosome 6q23.3 spanning less than 500 Kb in high grade invasive epithelial ovarian cancer. / Shridhar, Vijayalakshmi; Staub, Julie; Huntley, Brenda; Cliby, William Arthur; Jenkins, Robert Brian; Pass, Harvey I.; Hartmann, Lynn; Smith, David I.

In: Oncogene, Vol. 18, No. 26, 01.07.1999, p. 3913-3918.

Research output: Contribution to journalArticle

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abstract = "Detailed deletion mapping of chromosome 6q sequences in invasive ovarian tumors have implicated several broad regions involving 6q14-16, 6q21-23, 6q25-26, and the telomeric portion in band 6q27 as regions of frequent loss in this malignancy. In order to define regions of loss involved in the development of ovarian cancer, we used 23 polymorphic markers on 6q to examine allelic loss in 25 high-grade, late stage ovarian tumors. Four non-overlapping deletion regions were observed: (1) at 6q21-22.3 (D6S301-D6S292); (2) within a 1 cM region at 23.2-23.3 between markers D6S978-D6S1637 (at D6S311); (3) at 6q26 (between markers D6S411-D6S1277) and (4) at 6q27 with the markers D6S297 and D6S193. The highest region of loss was observed with marker D6S311 (lost in 17 of 19 informative cases, 89{\%}) in 6q23.3, followed by D6S977 and D6S1637 (71 and 55{\%}, respectively). The average fractional allele loss in the high-grade tumors was around 35{\%}. Previous reports have shown 6q27 as the region of most frequent loss in invasive ovarian cancer. However, our results indicate a novel region in 6q23.3 (spanning less than 500 Kb distance between the markers) with the highest loss, implicating this region of chromosome 6q to harbor a putative tumor suppressor gene involved in the development of invasive epithelial ovarian cancer.",
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