A novel rat model to study the role of intracranial pressure modulation on optic neuropathies

Uttio Roy Chowdhury, Bradley H. Holman, Michael P Fautsch

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Reduced intracranial pressure is considered a risk factor for glaucomatous optic neuropathies. All current data supporting intracranial pressure as a glaucoma risk factor comes from retrospective and prospective studies. Unfortunately, there are no relevant animal models for investigating this link experimentally. Here we report a novel rat model that can be used to study the role of intracranial pressure modulation on optic neuropathies. Stainless steel cannulae were inserted into the cisterna magna or the lateral ventricle of Sprague-Dawley and Brown Norway rats. The cannula was attached to a pressure transducer connected to a computer that recorded intracranial pressure in real-time. Intracranial pressure was modulated manually by adjusting the height of a column filled with artificial cerebrospinal fluid in relation to the animal's head. After data collection the morphological appearance of the brain tissue was analyzed. Based on ease of surgery and ability to retain the cannula, Brown Norway rats with the cannula implanted in the lateral ventricle were selected for further studies. Baseline intracranial pressure for rats was 5.5±1.5 cm water (n=5). Lowering of the artificial cerebrospinal fluid column by 2 cm and 4 cm below head level reduced ICP to 3.7±1.0 cm water (n=5) and 1.5±0.6 cm water (n=4), a reduction of 33.0% and 72.7% below baseline. Raising the cerebrospinal fluid column by 4 cm increased ICP to 7.5±1.4 cm water (n=2) corresponding to a 38.3% increase in intracranial pressure. Histological studies confirmed correct cannula placement and indicated minimal invasive damage to brain tissues. Our data suggests that the intraventricular cannula model is a unique and viable model that can be used to study the effect of altered intracranial pressure on glaucomatous optic neuropathies.

Original languageEnglish (US)
Article numbere82151
JournalPLoS One
Volume8
Issue number12
DOIs
StatePublished - Dec 18 2013

Fingerprint

Optic Nerve Diseases
peripheral nervous system diseases
Intracranial Pressure
optics
Rats
Optics
animal models
Modulation
cannulas
Cerebrospinal fluid
Cerebrospinal Fluid
cerebrospinal fluid
Water
Lateral Ventricles
Rattus norvegicus
Head
Brain
Animals
Cisterna Magna
Pressure Transducers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A novel rat model to study the role of intracranial pressure modulation on optic neuropathies. / Chowdhury, Uttio Roy; Holman, Bradley H.; Fautsch, Michael P.

In: PLoS One, Vol. 8, No. 12, e82151, 18.12.2013.

Research output: Contribution to journalArticle

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