A novel packaging system for the generation of helper-free oncolytic MVM vector stocks

A. Brandenburger, S. Russell

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

MVM-based autonomous parvoviral vectors have been shown to target the expression of heterologous genes in neoplastic cells and are therefore of interest for cancer gene therapy. The traditional method for production of parvoviral vectors requires the cotransfection of vector and helper plasmids into MVM-permissive cell lines, but recombination between the cotransfected plasmids invariably gives rise to vector stocks that are heavily contaminated with wild-type MVM. Therefore, to minimise recombination between the vector and helper genomes we have utilised a cell line in which the MVM helper functions are expressed inducibly from a modified MVM genome that is stably integrated into the host cell chromosome. Using this MVM packaging cell line, we could reproducibly generate MVM vector stocks that contained no detectable helper virus.

Original languageEnglish (US)
Pages (from-to)927-931
Number of pages5
JournalGene Therapy
Volume3
Issue number10
StatePublished - Jan 1 1996

Keywords

  • Cancer gene therapy
  • MVM
  • Packaging cell line
  • Parvoviral vector

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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