A novel life cycle modeling system for Ebola virus shows a genome length-dependent role of VP24 in virus infectivity

Ari Watt, Felicien Moukambi, Logan Banadyga, Allison Groseth, Julie Callison, Astrid Herwig, Hideki Ebihara, Heinz Feldmann, Thomas Hoenen

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Work with infectious Ebola viruses is restricted to biosafety level 4 (BSL4) laboratories, presenting a significant barrier for studying these viruses. Life cycle modeling systems, including minigenome systems and transcription- and replication-competent virus-like particle (trVLP) systems, allow modeling of the virus life cycle under BSL2 conditions; however, all current systems model only certain aspects of the virus life cycle, rely on plasmid-based viral protein expression, and have been used to model only single infectious cycles. We have developed a novel life cycle modeling system allowing continuous passaging of infectious trVLPs containing a tetracistronic minigenome that encodes a reporter and the viral proteins VP40, VP24, and GP1,2. This system is ideally suited for studying morphogenesis, budding, and entry, in addition to genome replication and transcription. Importantly, the specific infectivity of trVLPs in this system was~500-fold higher than that in previous systems. Using this system for functional studies of VP24, we showed that, contrary to previous reports, VP24 only very modestly inhibits genome replication and transcription when expressed in a regulated fashion, which we confirmed using infectious Ebola viruses. Interestingly, we also discovered a genome length-dependent effect of VP24 on particle infectivity, which was previously undetected due to the short length of monocistronic minigenomes and which is due at least partially to a previously unknown function of VP24 in RNA packaging. Based on our findings, we propose a model for the function of VP24 that reconciles all currently available data regarding the role of VP24 in nucleocapsid assembly as well as genome replication and transcription.

Original languageEnglish (US)
Pages (from-to)10511-10524
Number of pages14
JournalJournal of Virology
Volume88
Issue number18
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Ebolavirus
Life Cycle Stages
life cycle (organisms)
pathogenicity
Genome
Viruses
viruses
genome
Viral Proteins
transcription (genetics)
viral proteins
Nucleocapsid
Product Packaging
Morphogenesis
Virion
genome assembly
nucleocapsid
biosafety
Plasmids
virus-like particles

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Watt, A., Moukambi, F., Banadyga, L., Groseth, A., Callison, J., Herwig, A., ... Hoenen, T. (2014). A novel life cycle modeling system for Ebola virus shows a genome length-dependent role of VP24 in virus infectivity. Journal of Virology, 88(18), 10511-10524. https://doi.org/10.1128/JVI.01272-14

A novel life cycle modeling system for Ebola virus shows a genome length-dependent role of VP24 in virus infectivity. / Watt, Ari; Moukambi, Felicien; Banadyga, Logan; Groseth, Allison; Callison, Julie; Herwig, Astrid; Ebihara, Hideki; Feldmann, Heinz; Hoenen, Thomas.

In: Journal of Virology, Vol. 88, No. 18, 01.01.2014, p. 10511-10524.

Research output: Contribution to journalArticle

Watt, A, Moukambi, F, Banadyga, L, Groseth, A, Callison, J, Herwig, A, Ebihara, H, Feldmann, H & Hoenen, T 2014, 'A novel life cycle modeling system for Ebola virus shows a genome length-dependent role of VP24 in virus infectivity', Journal of Virology, vol. 88, no. 18, pp. 10511-10524. https://doi.org/10.1128/JVI.01272-14
Watt, Ari ; Moukambi, Felicien ; Banadyga, Logan ; Groseth, Allison ; Callison, Julie ; Herwig, Astrid ; Ebihara, Hideki ; Feldmann, Heinz ; Hoenen, Thomas. / A novel life cycle modeling system for Ebola virus shows a genome length-dependent role of VP24 in virus infectivity. In: Journal of Virology. 2014 ; Vol. 88, No. 18. pp. 10511-10524.
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