TY - JOUR
T1 - A novel immediate early response gene, IEX-1, is induced by ultraviolet radiation in human keratinocytes
AU - Kumar, Rajiv
AU - Kobayashi, Teruaki
AU - Warner, Gina M.
AU - Wu, Yanhong
AU - Salisbury, Jeffrey L.
AU - Lingle, Wilma
AU - Pittelkow, Mark R.
N1 - Funding Information:
1To whom correspondence should be addressed at Mayo Clinic, 200 First St., SW, 911A Guggenheim Bldg., Rochester, MN 55905. Fax: 507-266-4710. E-mail: rkumar@mayo.edu. Supported by NIH Grant: DK 25409 (R.K.).
PY - 1998/12/18
Y1 - 1998/12/18
N2 - Skin cancer is the most common human malignancy and is strongly associated with exposure to ultraviolet radiation (UVR). Several mechanisms including an increase in immediate early gene activation have been postulated to be involved in UVR-mediated carcinogenesis. We show that in a dose-dependent manner, UVR induces the expression of messenger RNA of a novel immediate early response gene, IEX-1, in human keratinocytes. Human keratinocytes and mouse fibroblasts transfected with an expression plasmid for IEX-1 grow at a faster rate than keratinocytes transfected with a similar plasmid that does not contain the IEX-1 sequence. IEX-1 protein is localized predominantly in the nucleus of keratinocytes by fluorescent antibody methods and by examination of the location of a green fluorescence IEX-1 fusion protein. Epidermal growth factor (EGF), a major mitogen of keratinocytes, and a tumor-promoting phorbol ester increase IEX-1 mRNA expression. IEX-1 may play a role in keratinocyte proliferation especially following UVR.
AB - Skin cancer is the most common human malignancy and is strongly associated with exposure to ultraviolet radiation (UVR). Several mechanisms including an increase in immediate early gene activation have been postulated to be involved in UVR-mediated carcinogenesis. We show that in a dose-dependent manner, UVR induces the expression of messenger RNA of a novel immediate early response gene, IEX-1, in human keratinocytes. Human keratinocytes and mouse fibroblasts transfected with an expression plasmid for IEX-1 grow at a faster rate than keratinocytes transfected with a similar plasmid that does not contain the IEX-1 sequence. IEX-1 protein is localized predominantly in the nucleus of keratinocytes by fluorescent antibody methods and by examination of the location of a green fluorescence IEX-1 fusion protein. Epidermal growth factor (EGF), a major mitogen of keratinocytes, and a tumor-promoting phorbol ester increase IEX-1 mRNA expression. IEX-1 may play a role in keratinocyte proliferation especially following UVR.
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U2 - 10.1006/bbrc.1998.9692
DO - 10.1006/bbrc.1998.9692
M3 - Article
C2 - 9878538
AN - SCOPUS:0032545348
SN - 0006-291X
VL - 253
SP - 336
EP - 341
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -