Abstract
We have isolated a prostate-specific gene (NKX3.1) in humans that is homologous to the Drosophila NK homeobox gene family. Northern blot analyses indicate that this gene is expressed at high levels in adult prostate and at a much lower level in testis, but is expressed little or not at all in several other tissues. In an androgen-dependent prostate carcinoma line, LNCaP, NKX3.1 mRNA is expressed at a basal level that was increased markedly upon androgen stimulation; the NKX3.1 mRNA was undetectable in several other human tumor cell lines including two androgen-independent prostate carcinoma lines. The NKX3.1 gene maps to chromosome band 8p21, a region frequently reported to undergo a loss of heterozygosity associated with tissue dedifferentiation and loss of androgen responsiveness during the progression of prostate cancer. Based on these data we propose that NKX3.1 is a candidate gene for playing a role in the opposing processes of androgen-driven differentiation of prostatic tissue and loss of that differentiation during the progression of prostate cancer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 69-77 |
| Number of pages | 9 |
| Journal | Genomics |
| Volume | 43 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1 1997 |
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ASJC Scopus subject areas
- Genetics
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A novel human prostate-specific, androgen-regulated homeobox gene (NKX3.1) that maps to 8p21, a region frequently deleted in prostate cancer. / He, Wei Wu; Sciavolino, Peter J.; Wing, John; Augustus, Meena; Hudson, Peter; Meissner, P. Scott; Curtis, R. Thomas; Shell, Brenda K.; Bostwick, David G.; Tindall, Donald J.; Gelmann, Edward P.; Abate-Shen, Cory; Carter, Kenneth C.
In: Genomics, Vol. 43, No. 1, 01.07.1997, p. 69-77.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A novel human prostate-specific, androgen-regulated homeobox gene (NKX3.1) that maps to 8p21, a region frequently deleted in prostate cancer
AU - He, Wei Wu
AU - Sciavolino, Peter J.
AU - Wing, John
AU - Augustus, Meena
AU - Hudson, Peter
AU - Meissner, P. Scott
AU - Curtis, R. Thomas
AU - Shell, Brenda K.
AU - Bostwick, David G.
AU - Tindall, Donald J.
AU - Gelmann, Edward P.
AU - Abate-Shen, Cory
AU - Carter, Kenneth C.
PY - 1997/7/1
Y1 - 1997/7/1
N2 - We have isolated a prostate-specific gene (NKX3.1) in humans that is homologous to the Drosophila NK homeobox gene family. Northern blot analyses indicate that this gene is expressed at high levels in adult prostate and at a much lower level in testis, but is expressed little or not at all in several other tissues. In an androgen-dependent prostate carcinoma line, LNCaP, NKX3.1 mRNA is expressed at a basal level that was increased markedly upon androgen stimulation; the NKX3.1 mRNA was undetectable in several other human tumor cell lines including two androgen-independent prostate carcinoma lines. The NKX3.1 gene maps to chromosome band 8p21, a region frequently reported to undergo a loss of heterozygosity associated with tissue dedifferentiation and loss of androgen responsiveness during the progression of prostate cancer. Based on these data we propose that NKX3.1 is a candidate gene for playing a role in the opposing processes of androgen-driven differentiation of prostatic tissue and loss of that differentiation during the progression of prostate cancer.
AB - We have isolated a prostate-specific gene (NKX3.1) in humans that is homologous to the Drosophila NK homeobox gene family. Northern blot analyses indicate that this gene is expressed at high levels in adult prostate and at a much lower level in testis, but is expressed little or not at all in several other tissues. In an androgen-dependent prostate carcinoma line, LNCaP, NKX3.1 mRNA is expressed at a basal level that was increased markedly upon androgen stimulation; the NKX3.1 mRNA was undetectable in several other human tumor cell lines including two androgen-independent prostate carcinoma lines. The NKX3.1 gene maps to chromosome band 8p21, a region frequently reported to undergo a loss of heterozygosity associated with tissue dedifferentiation and loss of androgen responsiveness during the progression of prostate cancer. Based on these data we propose that NKX3.1 is a candidate gene for playing a role in the opposing processes of androgen-driven differentiation of prostatic tissue and loss of that differentiation during the progression of prostate cancer.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031194107&partnerID=8YFLogxK
U2 - 10.1006/geno.1997.4715
DO - 10.1006/geno.1997.4715
M3 - Article
C2 - 9226374
AN - SCOPUS:0031194107
VL - 43
SP - 69
EP - 77
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 1
ER -