A novel enhancer regulates MGMT expression and promotes temozolomide resistance in glioblastoma

Xiaoyue Chen, Minjie Zhang, Haiyun Gan, Heping Wang, Jeong Heon Lee, Dong Fang, Gaspar J. Kitange, Lihong He, Zeng Hu, Ian F Parney, Fredric B. Meyer, Caterina Giannini, Jann N Sarkaria, Zhiguo Zhang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Temozolomide (TMZ) was used for the treatment of glioblastoma (GBM) for over a decade, but its treatment benefits are limited by acquired resistance, a process that remains incompletely understood. Here we report that an enhancer, located between the promoters of marker of proliferation Ki67 (MKI67) and O6-methylguanine-DNA-methyltransferase (MGMT) genes, is activated in TMZ-resistant patient-derived xenograft (PDX) lines and recurrent tumor samples. Activation of the enhancer correlates with increased MGMT expression, a major known mechanism for TMZ resistance. We show that forced activation of the enhancer in cell lines with low MGMT expression results in elevated MGMT expression. Deletion of this enhancer in cell lines with high MGMT expression leads to a dramatic reduction of MGMT and a lesser extent of Ki67 expression, increased TMZ sensitivity, and impaired proliferation. Together, these studies uncover a mechanism that regulates MGMT expression, confers TMZ resistance, and potentially regulates tumor proliferation.

Original languageEnglish (US)
Article number2949
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

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temozolomide
Methyltransferases
Glioblastoma
deoxyribonucleic acid
DNA
cultured cells
Tumors
tumors
Chemical activation
Cells
activation
Cell Line
deletion
Heterografts
genes
markers
Neoplasms

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

A novel enhancer regulates MGMT expression and promotes temozolomide resistance in glioblastoma. / Chen, Xiaoyue; Zhang, Minjie; Gan, Haiyun; Wang, Heping; Lee, Jeong Heon; Fang, Dong; Kitange, Gaspar J.; He, Lihong; Hu, Zeng; Parney, Ian F; Meyer, Fredric B.; Giannini, Caterina; Sarkaria, Jann N; Zhang, Zhiguo.

In: Nature Communications, Vol. 9, No. 1, 2949, 01.12.2018.

Research output: Contribution to journalArticle

Chen, Xiaoyue ; Zhang, Minjie ; Gan, Haiyun ; Wang, Heping ; Lee, Jeong Heon ; Fang, Dong ; Kitange, Gaspar J. ; He, Lihong ; Hu, Zeng ; Parney, Ian F ; Meyer, Fredric B. ; Giannini, Caterina ; Sarkaria, Jann N ; Zhang, Zhiguo. / A novel enhancer regulates MGMT expression and promotes temozolomide resistance in glioblastoma. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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AU - Fang, Dong

AU - Kitange, Gaspar J.

AU - He, Lihong

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AU - Parney, Ian F

AU - Meyer, Fredric B.

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AU - Zhang, Zhiguo

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