A novel animal model of human breast cancer metastasis to the spine: A pilot study using intracardiac injection and luciferase-expressing cells

Patricia Zadnik, Rachel Sarabia Estrada, Mari L. Groves, Camilo Molina, Christopher Jackson, Edward McCarthy, Ziya L. Gokaslan, Ali Bydon, Jean Paul Wolinsky, Timothy F. Witham, Daniel M. Sciubb

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Object. Metastatic spine disease is prevalent in cancer victims; 10%-30% of the 1.2 million new patients diagnosed with cancer in the US exhibit spinal metastases. Unfortunately, treatments are limited for these patients, as disseminated disease is often refractory to chemotherapy and is difficult to treat with surgical intervention alone. New animal models that accurately recapitulate the human disease process are needed to study the behavior of metastases in real time. Methods. In this study the authors report on a cell line that reliably generates bony metastases following intracardiac injection and can be tracked in real time using optical bioluminescence imaging. This line, RBC3, was derived from a metastatic breast adenocarcinoma lesion arising in the osseous spine of a rat following intracardiac injection of MDA-231 human breast cancer cells. Results. Upon culture and reinjection of RBC3, a statistically significantly increased systemic burden of metastatic tumor was noted. The resultant spine lesions were osteolytic, as demonstrated by small animal CT scanning. Conclusions. This cell line generates spinal metastases that can be tracked in real time and may serve as a useful tool in the study of metastatic disease in the spine.

Original languageEnglish (US)
Pages (from-to)217-225
Number of pages9
JournalJournal of Neurosurgery: Spine
Volume18
Issue number3
DOIs
StatePublished - Mar 2013
Externally publishedYes

Fingerprint

Luciferases
Spine
Animal Models
Breast Neoplasms
Neoplasm Metastasis
Injections
Cell Line
Optical Imaging
Tumor Burden
Neoplasms
Adenocarcinoma
Breast
Drug Therapy
Therapeutics

Keywords

  • Breast cancer
  • Metastasis
  • Oncology
  • Spine
  • Tumor

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery
  • Neurology

Cite this

A novel animal model of human breast cancer metastasis to the spine : A pilot study using intracardiac injection and luciferase-expressing cells. / Zadnik, Patricia; Sarabia Estrada, Rachel; Groves, Mari L.; Molina, Camilo; Jackson, Christopher; McCarthy, Edward; Gokaslan, Ziya L.; Bydon, Ali; Wolinsky, Jean Paul; Witham, Timothy F.; Sciubb, Daniel M.

In: Journal of Neurosurgery: Spine, Vol. 18, No. 3, 03.2013, p. 217-225.

Research output: Contribution to journalArticle

Zadnik, P, Sarabia Estrada, R, Groves, ML, Molina, C, Jackson, C, McCarthy, E, Gokaslan, ZL, Bydon, A, Wolinsky, JP, Witham, TF & Sciubb, DM 2013, 'A novel animal model of human breast cancer metastasis to the spine: A pilot study using intracardiac injection and luciferase-expressing cells', Journal of Neurosurgery: Spine, vol. 18, no. 3, pp. 217-225. https://doi.org/10.3171/2012.11.SPINE12325)
Zadnik, Patricia ; Sarabia Estrada, Rachel ; Groves, Mari L. ; Molina, Camilo ; Jackson, Christopher ; McCarthy, Edward ; Gokaslan, Ziya L. ; Bydon, Ali ; Wolinsky, Jean Paul ; Witham, Timothy F. ; Sciubb, Daniel M. / A novel animal model of human breast cancer metastasis to the spine : A pilot study using intracardiac injection and luciferase-expressing cells. In: Journal of Neurosurgery: Spine. 2013 ; Vol. 18, No. 3. pp. 217-225.
@article{8df5ace7b6e849559853e6f12080c705,
title = "A novel animal model of human breast cancer metastasis to the spine: A pilot study using intracardiac injection and luciferase-expressing cells",
abstract = "Object. Metastatic spine disease is prevalent in cancer victims; 10{\%}-30{\%} of the 1.2 million new patients diagnosed with cancer in the US exhibit spinal metastases. Unfortunately, treatments are limited for these patients, as disseminated disease is often refractory to chemotherapy and is difficult to treat with surgical intervention alone. New animal models that accurately recapitulate the human disease process are needed to study the behavior of metastases in real time. Methods. In this study the authors report on a cell line that reliably generates bony metastases following intracardiac injection and can be tracked in real time using optical bioluminescence imaging. This line, RBC3, was derived from a metastatic breast adenocarcinoma lesion arising in the osseous spine of a rat following intracardiac injection of MDA-231 human breast cancer cells. Results. Upon culture and reinjection of RBC3, a statistically significantly increased systemic burden of metastatic tumor was noted. The resultant spine lesions were osteolytic, as demonstrated by small animal CT scanning. Conclusions. This cell line generates spinal metastases that can be tracked in real time and may serve as a useful tool in the study of metastatic disease in the spine.",
keywords = "Breast cancer, Metastasis, Oncology, Spine, Tumor",
author = "Patricia Zadnik and {Sarabia Estrada}, Rachel and Groves, {Mari L.} and Camilo Molina and Christopher Jackson and Edward McCarthy and Gokaslan, {Ziya L.} and Ali Bydon and Wolinsky, {Jean Paul} and Witham, {Timothy F.} and Sciubb, {Daniel M.}",
year = "2013",
month = "3",
doi = "10.3171/2012.11.SPINE12325)",
language = "English (US)",
volume = "18",
pages = "217--225",
journal = "Journal of Neurosurgery: Spine",
issn = "1547-5654",
publisher = "American Association of Neurological Surgeons",
number = "3",

}

TY - JOUR

T1 - A novel animal model of human breast cancer metastasis to the spine

T2 - A pilot study using intracardiac injection and luciferase-expressing cells

AU - Zadnik, Patricia

AU - Sarabia Estrada, Rachel

AU - Groves, Mari L.

AU - Molina, Camilo

AU - Jackson, Christopher

AU - McCarthy, Edward

AU - Gokaslan, Ziya L.

AU - Bydon, Ali

AU - Wolinsky, Jean Paul

AU - Witham, Timothy F.

AU - Sciubb, Daniel M.

PY - 2013/3

Y1 - 2013/3

N2 - Object. Metastatic spine disease is prevalent in cancer victims; 10%-30% of the 1.2 million new patients diagnosed with cancer in the US exhibit spinal metastases. Unfortunately, treatments are limited for these patients, as disseminated disease is often refractory to chemotherapy and is difficult to treat with surgical intervention alone. New animal models that accurately recapitulate the human disease process are needed to study the behavior of metastases in real time. Methods. In this study the authors report on a cell line that reliably generates bony metastases following intracardiac injection and can be tracked in real time using optical bioluminescence imaging. This line, RBC3, was derived from a metastatic breast adenocarcinoma lesion arising in the osseous spine of a rat following intracardiac injection of MDA-231 human breast cancer cells. Results. Upon culture and reinjection of RBC3, a statistically significantly increased systemic burden of metastatic tumor was noted. The resultant spine lesions were osteolytic, as demonstrated by small animal CT scanning. Conclusions. This cell line generates spinal metastases that can be tracked in real time and may serve as a useful tool in the study of metastatic disease in the spine.

AB - Object. Metastatic spine disease is prevalent in cancer victims; 10%-30% of the 1.2 million new patients diagnosed with cancer in the US exhibit spinal metastases. Unfortunately, treatments are limited for these patients, as disseminated disease is often refractory to chemotherapy and is difficult to treat with surgical intervention alone. New animal models that accurately recapitulate the human disease process are needed to study the behavior of metastases in real time. Methods. In this study the authors report on a cell line that reliably generates bony metastases following intracardiac injection and can be tracked in real time using optical bioluminescence imaging. This line, RBC3, was derived from a metastatic breast adenocarcinoma lesion arising in the osseous spine of a rat following intracardiac injection of MDA-231 human breast cancer cells. Results. Upon culture and reinjection of RBC3, a statistically significantly increased systemic burden of metastatic tumor was noted. The resultant spine lesions were osteolytic, as demonstrated by small animal CT scanning. Conclusions. This cell line generates spinal metastases that can be tracked in real time and may serve as a useful tool in the study of metastatic disease in the spine.

KW - Breast cancer

KW - Metastasis

KW - Oncology

KW - Spine

KW - Tumor

UR - http://www.scopus.com/inward/record.url?scp=84874686483&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874686483&partnerID=8YFLogxK

U2 - 10.3171/2012.11.SPINE12325)

DO - 10.3171/2012.11.SPINE12325)

M3 - Article

C2 - 23259542

AN - SCOPUS:84874686483

VL - 18

SP - 217

EP - 225

JO - Journal of Neurosurgery: Spine

JF - Journal of Neurosurgery: Spine

SN - 1547-5654

IS - 3

ER -