A noninvasive ‘swish and spit’ method for collecting nucleated cells for hla typing by pcr in population studies

Mary S. Hayney, Gregory A. Poland, James J. Lipsky

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Buccal-cell-derived DNA collected by a ‘swish and spit’ technique and blood-derived DNA were compared for ease of collection, participant acceptance, utility and accuracy for HLA class II DR typing by the polymerase chain reaction with sequence-specific primers (PCR-SSP). The HLA class II DR typing results determined from DNA extracted by proteinase K digestion followed by phenol/chloroform extraction and ethanol precipitation from buccal cells and blood cells were identical for all subjects we studied (n = 10). Class II typing by PCR-SSP using DNA extracted from buccal cells stored at -20, 4, 25 or 37°C for 1 week was successful. The samples can be collected without medical supervision and are not affected by exposure to a variety of temperature conditions for up to 1 week. The stability of the buccal cell specimens to these extreme conditions demonstrates the utility of this ‘swish and spit’ technique for collecting nucleated cells for geographically dispersed large-scale population studies. Buccal-cell-derived DNA collected by a simple ‘swish and spit’ mouthwash technique is an excellent and practical substitute for blood-derived DNA.

Original languageEnglish (US)
Pages (from-to)108-111
Number of pages4
JournalHuman Heredity
Volume46
Issue number2
DOIs
StatePublished - Jan 1996

Keywords

  • Blood
  • Buccal cells
  • DNA
  • HLA
  • Mouthwash

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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