A noninvasive murine model of hind limb ischemia-reperfusion injury

James A. Bonheur, Hassan Albadawi, George M. Patton, Michael T. Watkins

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Background. This study describes a novel murine method of the Controlled Tension Tourniquet (CTT). The CTT applies a measured circumferential tension to hind limbs using a tourniquet attached to digital strain gauges, and is useful for investigating hind limb ischemia reperfusion (IR). Materials and methods. Mice were subjected to 1, 3, or 6 h of unilateral hind limb ischemia followed by either 4 or 24 h of reperfusion. Blood flow in the ischemic, reperfused, and contralateral limbs was monitored using a Laser Doppler Imager. Edema in the IR limbs was documented by changes in the wet weight to dry weight ratio. Myeloperoxidase and tetrazolium based mitochondrial activity assays indicated neutrophil infiltration and tissue viability, respectively. Results. During reperfusion following 1, 4, or 6 h, flow stabilized at 100%, 53%, and 23% of baseline levels, respectively. Edema was present all in IR limbs after 4 h of reperfusion, but increased with the duration of ischemia. After 24 h of reperfusion neutrophil infiltration was equivalent in all IR limbs after all intervals of ischemia. After 24 h of reperfusion, tissue viability after 1 h of ischemia was equivalent to sham or contralateral limbs. At 3 or 6 h of ischemia and 24 h reperfusion decreased tissue viability to 40% of sham and contralateral limbs. Conclusions. The CTT provides a reproducible, noninvasive model of acute limb ischemia, which reflects the biochemical indices of microvascular injury, inflammation and flow characteristic of reperfusion injury.

Original languageEnglish (US)
Pages (from-to)55-63
Number of pages9
JournalJournal of Surgical Research
Volume116
Issue number1
DOIs
StatePublished - Jan 1 2004
Externally publishedYes

Fingerprint

Reperfusion Injury
Reperfusion
Ischemia
Extremities
Tourniquets
Tissue Survival
Neutrophil Infiltration
Edema
Weights and Measures
Peroxidase
Lasers
Inflammation
Wounds and Injuries

Keywords

  • Controlled tension tourniquet
  • Ischemia
  • Laser Doppler imager
  • Myeloperoxidase
  • Reperfusion

ASJC Scopus subject areas

  • Surgery

Cite this

A noninvasive murine model of hind limb ischemia-reperfusion injury. / Bonheur, James A.; Albadawi, Hassan; Patton, George M.; Watkins, Michael T.

In: Journal of Surgical Research, Vol. 116, No. 1, 01.01.2004, p. 55-63.

Research output: Contribution to journalArticle

Bonheur, James A. ; Albadawi, Hassan ; Patton, George M. ; Watkins, Michael T. / A noninvasive murine model of hind limb ischemia-reperfusion injury. In: Journal of Surgical Research. 2004 ; Vol. 116, No. 1. pp. 55-63.
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