A new model for dermatitis herpetiformis that uses HLA-DQ8 transgenic NOD mice

Eric Marietta, Kay Black, Michael Camilleri, Patricia Krause, Roy S. Rogers, Chella David, Mark R. Pittelkow, Joseph A Murray

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Dermatitis herpetiformis (DH) is an autoimmune blistering skin disorder that is associated with gluten sensitivity. It presents as a papulovesicular rash and is often associated with enteropathy. The rash resolves when the patient is placed on a gluten-free diet and/or dapsone. DH, as well as celiac disease, is tightly associated with DQ2 and DQ8. A novel mouse model for DH is described that utilizes the NOD background and the HLA-DQ8 transgene. The addition of DQ8 contributes sensitivity to gliadin, and the addition of the NOD background contributes to autoimmunity and pathogenesis. Fifteen NOD DQ8 + mice of 90 that were sensitized to gluten developed blistering pathology similar to that seen in DH. Neutrophil infiltration of the dermis, deposition of IgA at the dermal-epidermal junction, and a complete reversal of the blistering phenomenon with the administration of a gluten-free diet with or without dapsone were observed. None of the 3 blistering mice examined had small-bowel pathology. This animal model of DH will be useful to determine the specificity of the IgA deposits, as well as the pathogenic mechanisms that occur in the skin as a result of gluten ingestion.

Original languageEnglish (US)
Pages (from-to)1090-1097
Number of pages8
JournalJournal of Clinical Investigation
Volume114
Issue number8
DOIs
StatePublished - Oct 2004

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Dermatitis Herpetiformis
Inbred NOD Mouse
Transgenic Mice
Glutens
Gluten-Free Diet
Dapsone
Exanthema
Immunoglobulin A
Skin
Pathology
Gliadin
Neutrophil Infiltration
Celiac Disease
Dermis
Autoimmunity
Transgenes
Animal Models
Eating
HLA-DQ8 antigen

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A new model for dermatitis herpetiformis that uses HLA-DQ8 transgenic NOD mice. / Marietta, Eric; Black, Kay; Camilleri, Michael; Krause, Patricia; Rogers, Roy S.; David, Chella; Pittelkow, Mark R.; Murray, Joseph A.

In: Journal of Clinical Investigation, Vol. 114, No. 8, 10.2004, p. 1090-1097.

Research output: Contribution to journalArticle

Marietta, E, Black, K, Camilleri, M, Krause, P, Rogers, RS, David, C, Pittelkow, MR & Murray, JA 2004, 'A new model for dermatitis herpetiformis that uses HLA-DQ8 transgenic NOD mice', Journal of Clinical Investigation, vol. 114, no. 8, pp. 1090-1097. https://doi.org/10.1172/JCI200421055
Marietta E, Black K, Camilleri M, Krause P, Rogers RS, David C et al. A new model for dermatitis herpetiformis that uses HLA-DQ8 transgenic NOD mice. Journal of Clinical Investigation. 2004 Oct;114(8):1090-1097. https://doi.org/10.1172/JCI200421055
Marietta, Eric ; Black, Kay ; Camilleri, Michael ; Krause, Patricia ; Rogers, Roy S. ; David, Chella ; Pittelkow, Mark R. ; Murray, Joseph A. / A new model for dermatitis herpetiformis that uses HLA-DQ8 transgenic NOD mice. In: Journal of Clinical Investigation. 2004 ; Vol. 114, No. 8. pp. 1090-1097.
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